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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5

Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342309     NCBI Gene Symbol: LOC103342309
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342309
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCCTT)2
Repeat start & end within CDS Repeat start: 29     Repeat end: 38
Forward primer Primer sequence:   TGTCGTGGTCAAGGGTTTGT     Tm(°C): 59.746     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCGCCAAATAGGACTACCG     Tm(°C): 59.896     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1     End: 165     Product size (bp): 165
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342309     NCBI Gene Symbol: LOC103342309
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342309
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TAG)3
Repeat start & end within CDS Repeat start: 114     Repeat end: 122
Forward primer Primer sequence:   TGTCGTGGTCAAGGGTTTGT     Tm(°C): 59.746     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCGCCAAATAGGACTACCG     Tm(°C): 59.896     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1     End: 165     Product size (bp): 165
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342309     NCBI Gene Symbol: LOC103342309
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342309
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GTTCA)2
Repeat start & end within CDS Repeat start: 466     Repeat end: 475
Forward primer Primer sequence:   CAGAAGATTGCTCGGCATGC     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGGCATGTCAATCTGCTCCC     Tm(°C): 60.107     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 411     End: 713     Product size (bp): 303
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342309     NCBI Gene Symbol: LOC103342309
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342309
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGTCCAACGTCGTGAGAGTG     Tm(°C): 59.97     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCAGCCACGTCAGCATTGA     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 386     End: 491     Product size (bp): 106
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329894     NCBI Gene Symbol: LOC103329894
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 21232063 ...... 21234752
CDS Sequence 103329894
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTA)3
Repeat start & end within CDS Repeat start: 113     Repeat end: 121
Forward primer Primer sequence:   AGGGTTTGCAAAAGAGCCCT     Tm(°C): 60.106     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CGAGCTTGAAGCCCCAACTA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 12     End: 177     Product size (bp): 166
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329894     NCBI Gene Symbol: LOC103329894
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 21232063 ...... 21234752
CDS Sequence 103329894
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGTTT)2
Repeat start & end within CDS Repeat start: 304     Repeat end: 313
Forward primer Primer sequence:   TGTTGAGAATCCAGCGACCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCTCCATACCCAGTAGCT     Tm(°C): 60.031     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 226     End: 387     Product size (bp): 162
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Enzyme Id:  K02258

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329894     NCBI Gene Symbol: LOC103329894
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase assembly protein COX11; mitochondrial-like      Other designations:   cytochrome c oxidase assembly protein COX11; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 21232063 ...... 21234752
CDS Sequence 103329894
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGTTGAGAATCCAGCGACCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCTCCATACCCAGTAGCT     Tm(°C): 60.031     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 226     End: 387     Product size (bp): 162
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