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Statistics

Number of enzymes: 1
Total Number of designed primers: 6
Number of PGTM primers:     1
Number of PMTM primers:     5

Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCACCT)2
Repeat start & end within CDS Repeat start: 151     Repeat end: 162
Forward primer Primer sequence:   GATTCTCGCCCAAGCTCGTA     Tm(°C): 59.898     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGTACGAAGCTTTGGAGGCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 19     End: 264     Product size (bp): 246
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Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCCAT)2
Repeat start & end within CDS Repeat start: 365     Repeat end: 374
Forward primer Primer sequence:   GCCACTATGGTCGCTGCTAT     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGCGTCCTGATGGAAGTGG     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 271     End: 514     Product size (bp): 244
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Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTG)3
Repeat start & end within CDS Repeat start: 545     Repeat end: 553
Forward primer Primer sequence:   CCACTTCCATCAGGACGCAT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCTGGGAGAAGCATTGCAT     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 495     End: 770     Product size (bp): 276
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Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATG)2
Repeat start & end within CDS Repeat start: 750     Repeat end: 759
Forward primer Primer sequence:   ATACATGGGTTGGTGCGGTT     Tm(°C): 59.961     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CCAGAAGCATCAGCAAGGGA     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 673     End: 877     Product size (bp): 205
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Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CCATTT)2
Repeat start & end within CDS Repeat start: 1279     Repeat end: 1290
Forward primer Primer sequence:   TCCATCAGGACCGCTCTACA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACATACGAAGGAACCGGGA     Tm(°C): 58.731     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1024     End: 1310     Product size (bp): 287
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Enzyme Id:  K02257
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103319063     NCBI Gene Symbol: LOC103319063
Gene Aliases
Gene description & Other designations Description:   protoheme IX farnesyltransferase; mitochondrial      Other designations:   protoheme IX farnesyltransferase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 1389863 ...... 1394920
CDS Sequence 103319063
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATGCAATGCTTCTCCCAGCT     Tm(°C): 60.033     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGTAGAGCGGTCCTGATGGA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 751     End: 1043     Product size (bp): 293
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