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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     2
Number of PMTM primers:     1

Enzyme Id:  K02153

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326564     NCBI Gene Symbol: LOC103326564
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit e1      Other designations:   V-type proton ATPase subunit e1|V-type proton ATPase subunit e
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 14732613 ...... 14735183
CDS Sequence 103326564
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTG)3
Repeat start & end within CDS Repeat start: 132     Repeat end: 140
Forward primer Primer sequence:   ACCAGAATTTGCTGCAACCG     Tm(°C): 59.685     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGAGTGGTTTCATTTGTGCAAG     Tm(°C): 57.956     GC (%): 40.909     Size: 22
Primer start, end within sequence and product size Start: 60     End: 183     Product size (bp): 124
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Enzyme Id:  K02153

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326564     NCBI Gene Symbol: LOC103326564
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit e1      Other designations:   V-type proton ATPase subunit e1|V-type proton ATPase subunit e
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 14732613 ...... 14735183
CDS Sequence 103326564
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGGGATCATTGCTTCTCTGTGT     Tm(°C): 59.693     GC (%): 45.455     Size: 22
Reverse primer Primer sequence:   ATTGCCCACATCATCCAGCA     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 38     End: 154     Product size (bp): 117
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Enzyme Id:  K02153

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326996     NCBI Gene Symbol: LOC103326996
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit e1-like      Other designations:   V-type proton ATPase subunit e1-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 20800968 ...... 20802687
CDS Sequence 103326996
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGGGCGTAATTGCTTCCCTT     Tm(°C): 59.961     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGAGCGGTTTCATCTGTGCA     Tm(°C): 60.25     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 37     End: 183     Product size (bp): 147
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