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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     3
Number of PMTM primers:     1

Enzyme Id:  K02150

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337692     NCBI Gene Symbol: LOC103337692
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit E-like      Other designations:   V-type proton ATPase subunit E-like
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 10422788 ...... 10425357
CDS Sequence 103337692
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GAA)3ttcaacattgagaagttgcagctggtcgaggctg(AGA)4
Repeat start & end within CDS Repeat start: 97     Repeat end: 151
Forward primer Primer sequence:   AAAGGCCAACGAGATCTCCG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACGTCGACTTGCTTCTCT     Tm(°C): 59.759     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 68     End: 192     Product size (bp): 125
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Enzyme Id:  K02150

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337692     NCBI Gene Symbol: LOC103337692
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit E-like      Other designations:   V-type proton ATPase subunit E-like
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 10422788 ...... 10425357
CDS Sequence 103337692
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAAGGCCAACGAGATCTCCG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGAAACAGCCTCCACCAGAT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 68     End: 437     Product size (bp): 370
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Enzyme Id:  K02150

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103338483     NCBI Gene Symbol: LOC103338483
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit E-like      Other designations:   V-type proton ATPase subunit E-like
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 14844180 ...... 14845814
CDS Sequence 103338483
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ACCAGCCCTTAAATCCCAGC     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGATACGGCGATCTCGTTGG     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 52     End: 158     Product size (bp): 107
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Enzyme Id:  K02150

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342694     NCBI Gene Symbol: LOC103342694
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit E2      Other designations:   V-type proton ATPase subunit E2
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 22607070 ...... 22608828
CDS Sequence 103342694
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAGAGGCCGAAGAGAAAGCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGTCATCCGAAACACGCAGA     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 55     End: 318     Product size (bp): 264
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