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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K02146

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329976     NCBI Gene Symbol: LOC103329976
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit d2      Other designations:   V-type proton ATPase subunit d2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 21717215 ...... 21720848
CDS Sequence 103329976
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TACAT)2atggccacatgatagacaatgttgtcctgattgttactggaaccttg
cat(GA)4
Repeat start & end within CDS Repeat start: 295     Repeat end: 362
Forward primer Primer sequence:   ATGAACCGTCCCCATTGCAT     Tm(°C): 60.033     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAAACATGCCCAAAGGGTGG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 178     End: 405     Product size (bp): 228
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Enzyme Id:  K02146

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329976     NCBI Gene Symbol: LOC103329976
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit d2      Other designations:   V-type proton ATPase subunit d2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 21717215 ...... 21720848
CDS Sequence 103329976
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCAACATTCACGGCGGGTA     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATGCAATGGGGACGGTTCAT     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 24     End: 197     Product size (bp): 174
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