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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K02145

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327431     NCBI Gene Symbol: LOC103327431
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase catalytic subunit A      Other designations:   V-type proton ATPase catalytic subunit A
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 1591011 ...... 1597067
CDS Sequence 103327431
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ATGGC)2
Repeat start & end within CDS Repeat start: 104     Repeat end: 113
Forward primer Primer sequence:   GAGTATGGCTACGTCCGCAA     Tm(°C): 59.898     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAAGGCCTCTGGATTCCGT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 57     End: 326     Product size (bp): 270
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Enzyme Id:  K02145

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327431     NCBI Gene Symbol: LOC103327431
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase catalytic subunit A      Other designations:   V-type proton ATPase catalytic subunit A
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 1591011 ...... 1597067
CDS Sequence 103327431
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AGGTGA)2
Repeat start & end within CDS Repeat start: 429     Repeat end: 440
Forward primer Primer sequence:   CCACGTGGTGTATCTGTCCC     Tm(°C): 60.108     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AGAACACGCTGTCCAGTGAG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 363     End: 706     Product size (bp): 344
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Enzyme Id:  K02145

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327431     NCBI Gene Symbol: LOC103327431
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase catalytic subunit A      Other designations:   V-type proton ATPase catalytic subunit A
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 1591011 ...... 1597067
CDS Sequence 103327431
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAAAT)2
Repeat start & end within CDS Repeat start: 848     Repeat end: 857
Forward primer Primer sequence:   TTCCTGGGGCTTTTGGTTGT     Tm(°C): 60.032     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GCTTCAGCCCATCGAGAAGT     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 748     End: 1078     Product size (bp): 331
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Enzyme Id:  K02145

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327431     NCBI Gene Symbol: LOC103327431
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase catalytic subunit A      Other designations:   V-type proton ATPase catalytic subunit A
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 1591011 ...... 1597067
CDS Sequence 103327431
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ACGGAATCCAGAGGCCTTTG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACAACCAAAAGCCCCAGGAA     Tm(°C): 60.032     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 307     End: 767     Product size (bp): 461
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