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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K02144

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103333478     NCBI Gene Symbol: LOC103333478
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit H      Other designations:   V-type proton ATPase subunit H
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 24699945 ...... 24704182
CDS Sequence 103333478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGAG)2
Repeat start & end within CDS Repeat start: 362     Repeat end: 371
Forward primer Primer sequence:   ACGGCCCTGCTTATGTTCAA     Tm(°C): 59.962     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACAGTGCCATCTTGGGGTTT     Tm(°C): 59.812     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 157     End: 427     Product size (bp): 271
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Enzyme Id:  K02144

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103333478     NCBI Gene Symbol: LOC103333478
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit H      Other designations:   V-type proton ATPase subunit H
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 24699945 ...... 24704182
CDS Sequence 103333478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCCTA)2
Repeat start & end within CDS Repeat start: 710     Repeat end: 719
Forward primer Primer sequence:   CCCAACTGCTGTCAATTGCC     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTCCCTTTGGAGAGCAAGT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 548     End: 854     Product size (bp): 307
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Enzyme Id:  K02144

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103333478     NCBI Gene Symbol: LOC103333478
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit H      Other designations:   V-type proton ATPase subunit H
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 24699945 ...... 24704182
CDS Sequence 103333478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTT)3
Repeat start & end within CDS Repeat start: 814     Repeat end: 822
Forward primer Primer sequence:   CCCAACTGCTGTCAATTGCC     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTCCCTTTGGAGAGCAAGT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 548     End: 854     Product size (bp): 307
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Enzyme Id:  K02144

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103333478     NCBI Gene Symbol: LOC103333478
Gene Aliases
Gene description & Other designations Description:   V-type proton ATPase subunit H      Other designations:   V-type proton ATPase subunit H
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 24699945 ...... 24704182
CDS Sequence 103333478
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AATGGCTTTGTGCACAGCTG     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CGTCCCTTTGGAGAGCAAGT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 499     End: 854     Product size (bp): 356
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