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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     3
Number of PMTM primers:     1

Enzyme Id:  K02140

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320535     NCBI Gene Symbol: LOC103320535
Gene Aliases
Gene description & Other designations Description:   uncharacterized LOC103320535      Other designations:   uncharacterized protein LOC103320535
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 11262938 ...... 11263883
CDS Sequence 103320535
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTG)3
Repeat start & end within CDS Repeat start: 320     Repeat end: 328
Forward primer Primer sequence:   GCTGGTTCAATTGCAAGGCA     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACCAGTGAATTTAAATCCCCGA     Tm(°C): 57.69     GC (%): 40.909     Size: 22
Primer start, end within sequence and product size Start: 14     End: 359     Product size (bp): 346
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Enzyme Id:  K02140

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320535     NCBI Gene Symbol: LOC103320535
Gene Aliases
Gene description & Other designations Description:   uncharacterized LOC103320535      Other designations:   uncharacterized protein LOC103320535
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 11262938 ...... 11263883
CDS Sequence 103320535
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCTGGTTCAATTGCAAGGCA     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AACCTCCTTCCGCAACGATT     Tm(°C): 59.964     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 14     End: 221     Product size (bp): 208
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Enzyme Id:  K02140

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340766     NCBI Gene Symbol: LOC103340766
Gene Aliases
Gene description & Other designations Description:   uncharacterized LOC103340766      Other designations:   uncharacterized protein LOC103340766
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14256297 ...... 14258610
CDS Sequence 103340766
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGCCAGTTGCAATCTAAGGC     Tm(°C): 59.901     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACTCCAGCCCAAACAAAGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 15     End: 301     Product size (bp): 287
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Enzyme Id:  K02140

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327731     NCBI Gene Symbol: LOC103327731
Gene Aliases
Gene description & Other designations Description:   uncharacterized LOC103327731      Other designations:   uncharacterized protein LOC103327731
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3658067 ...... 3660806
CDS Sequence 103327731
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGAAGGTGGTTCAGGCATCG     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCAGCAAGACCAGCATTCT     Tm(°C): 59.962     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 25     End: 281     Product size (bp): 257
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