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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     2
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 1

Enzyme Id:  K02134

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331497     NCBI Gene Symbol: LOC103331497
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit delta'; mitochondrial-like      Other designations:   ATP synthase subunit delta'; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12348851 ...... 12352361
CDS Sequence 103331497
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGCTC)2
Repeat start & end within CDS Repeat start: 512     Repeat end: 521
Forward primer Primer sequence:   AGAAGGGGCTTGCAGAGTTC     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCGGCATTGAGGGCACTAT     Tm(°C): 60.527     GC (%): 57.895     Size: 19
Primer start, end within sequence and product size Start: 484     End: 589     Product size (bp): 106
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Enzyme Id:  K02134

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331497     NCBI Gene Symbol: LOC103331497
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit delta'; mitochondrial-like      Other designations:   ATP synthase subunit delta'; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12348851 ...... 12352361
CDS Sequence 103331497
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCGCC)2
Repeat start & end within CDS Repeat start: 6     Repeat end: 15
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K02134

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331497     NCBI Gene Symbol: LOC103331497
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit delta'; mitochondrial-like      Other designations:   ATP synthase subunit delta'; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12348851 ...... 12352361
CDS Sequence 103331497
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAACTCCAGTAGCCGACACG     Tm(°C): 60.039     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCTTCTGGGTGAACTCTGC     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 79     End: 514     Product size (bp): 436
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Enzyme Id:  K02134

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328881     NCBI Gene Symbol: LOC103328881
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit delta'; mitochondrial      Other designations:   ATP synthase subunit delta'; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 14848019 ...... 14849962
CDS Sequence 103328881
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAAGGGAGCGAAGTGACCAA     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGGCTGAACTGAGCTTCTGG     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 369     End: 534     Product size (bp): 166
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