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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     1
Number of PMTM primers:     4

Enzyme Id:  K02126

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335303     NCBI Gene Symbol: LOC103335303
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit a      Other designations:   ATP synthase subunit a
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 8371988 ...... 8374406
CDS Sequence 103335303
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GGA)3
Repeat start & end within CDS Repeat start: 166     Repeat end: 174
Forward primer Primer sequence:   CCGCTTGAGCAATTTGCCAT     Tm(°C): 60.109     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTACCGAGGATTGCCAAGCA     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 27     End: 207     Product size (bp): 181
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Enzyme Id:  K02126

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335303     NCBI Gene Symbol: LOC103335303
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit a      Other designations:   ATP synthase subunit a
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 8371988 ...... 8374406
CDS Sequence 103335303
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ACTTTT)2
Repeat start & end within CDS Repeat start: 304     Repeat end: 315
Forward primer Primer sequence:   TGCTTGGCAATCCTCGGTAG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACTCCTGCGGGTAATGAG     Tm(°C): 59.895     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 188     End: 480     Product size (bp): 293
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Enzyme Id:  K02126

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335303     NCBI Gene Symbol: LOC103335303
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit a      Other designations:   ATP synthase subunit a
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 8371988 ...... 8374406
CDS Sequence 103335303
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTATG)2
Repeat start & end within CDS Repeat start: 620     Repeat end: 629
Forward primer Primer sequence:   TCGCGCATTAAGCTCAGGAA     Tm(°C): 60.108     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTCTTCTCGGTCCGCTTTGG     Tm(°C): 60.32     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 533     End: 850     Product size (bp): 318
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Enzyme Id:  K02126

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335303     NCBI Gene Symbol: LOC103335303
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit a      Other designations:   ATP synthase subunit a
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 8371988 ...... 8374406
CDS Sequence 103335303
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAGAA)2
Repeat start & end within CDS Repeat start: 895     Repeat end: 904
Forward primer Primer sequence:   GGGTTCGCTTGGACTATGCT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGGCGAGCCGAACTAAAGG     Tm(°C): 60.462     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 606     End: 926     Product size (bp): 321
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Enzyme Id:  K02126

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335303     NCBI Gene Symbol: LOC103335303
Gene Aliases
Gene description & Other designations Description:   ATP synthase subunit a      Other designations:   ATP synthase subunit a
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 8371988 ...... 8374406
CDS Sequence 103335303
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGCTTGGCAATCCTCGGTAG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACTCCTGCGGGTAATGAG     Tm(°C): 59.895     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 188     End: 480     Product size (bp): 293
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