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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5

Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324541     NCBI Gene Symbol: LOC103324541
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic      Other designations:   ATP synthase gamma chain; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 969288 ...... 970895
CDS Sequence 103324541
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GT)5ggatgctgc(TGA)3gttcttcaggttgacaacaaa(GGAAG)2ttgact
gt(AG)4
Repeat start & end within CDS Repeat start: 767     Repeat end: 841
Forward primer Primer sequence:   CCCAACTGCCAAAGAAGCAC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCTCGAATTGCAAAACCGGT     Tm(°C): 60.04     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 590     End: 891     Product size (bp): 302
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324541     NCBI Gene Symbol: LOC103324541
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic      Other designations:   ATP synthase gamma chain; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 969288 ...... 970895
CDS Sequence 103324541
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1035     Repeat end: 1043
Forward primer Primer sequence:   ACTAGCCAGTGAGCTTGCTG     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTGCCTGTTGTAGACCCTGG     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 968     End: 1067     Product size (bp): 100
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324541     NCBI Gene Symbol: LOC103324541
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic      Other designations:   ATP synthase gamma chain; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 969288 ...... 970895
CDS Sequence 103324541
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGAGCAGCTCCAAGTGGAAG     Tm(°C): 59.964     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTGCTTCTTTGGCAGTTGGG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 326     End: 609     Product size (bp): 284
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326447     NCBI Gene Symbol: LOC103326447
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic-like      Other designations:   ATP synthase gamma chain; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 13920837 ...... 13922525
CDS Sequence 103326447
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGGA)2
Repeat start & end within CDS Repeat start: 470     Repeat end: 479
Forward primer Primer sequence:   AAGCAGTCATCAATGGCCGA     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACCCTTTTTGCCGACACTGA     Tm(°C): 60.107     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 247     End: 509     Product size (bp): 263
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326447     NCBI Gene Symbol: LOC103326447
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic-like      Other designations:   ATP synthase gamma chain; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 13920837 ...... 13922525
CDS Sequence 103326447
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CAA)3
Repeat start & end within CDS Repeat start: 575     Repeat end: 583
Forward primer Primer sequence:   TCAGTGTCGGCAAAAAGGGT     Tm(°C): 60.107     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCCACACTCAGTTTGCCTCC     Tm(°C): 59.891     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 490     End: 817     Product size (bp): 328
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326447     NCBI Gene Symbol: LOC103326447
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic-like      Other designations:   ATP synthase gamma chain; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 13920837 ...... 13922525
CDS Sequence 103326447
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ATGGGA)2
Repeat start & end within CDS Repeat start: 740     Repeat end: 751
Forward primer Primer sequence:   TCAGTGTCGGCAAAAAGGGT     Tm(°C): 60.107     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCCACACTCAGTTTGCCTCC     Tm(°C): 59.891     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 490     End: 817     Product size (bp): 328
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Enzyme Id:  K02115

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326447     NCBI Gene Symbol: LOC103326447
Gene Aliases
Gene description & Other designations Description:   ATP synthase gamma chain; chloroplastic-like      Other designations:   ATP synthase gamma chain; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 13920837 ...... 13922525
CDS Sequence 103326447
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGGGCTTTCACCGGTTATGG     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTTAGTGCCTCAGCTCCAGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 845     End: 1108     Product size (bp): 264
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