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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     1
Number of PMTM primers:     4

Enzyme Id:  K02111
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668002     NCBI Gene Symbol: atpA
Gene Aliases CP95_p082
Gene description & Other designations Description:   ATP synthase CF1 alpha subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 10070 ...... 11593
CDS Sequence 18668002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GTACT)2
Repeat start & end within CDS Repeat start: 89     Repeat end: 98
Forward primer Primer sequence:   ATCCGCGAACGTATTGAGCA     Tm(°C): 60.179     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTCGCCCCGACCATCAATAG     Tm(°C): 59.894     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 36     End: 362     Product size (bp): 327
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Enzyme Id:  K02111
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668002     NCBI Gene Symbol: atpA
Gene Aliases CP95_p082
Gene description & Other designations Description:   ATP synthase CF1 alpha subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 10070 ...... 11593
CDS Sequence 18668002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CTCCTG)2
Repeat start & end within CDS Repeat start: 395     Repeat end: 406
Forward primer Primer sequence:   CTATTGATGGTCGGGGCGAA     Tm(°C): 59.894     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTAAAGCGGTCACCACCTGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 343     End: 639     Product size (bp): 297
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Enzyme Id:  K02111
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668002     NCBI Gene Symbol: atpA
Gene Aliases CP95_p082
Gene description & Other designations Description:   ATP synthase CF1 alpha subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 10070 ...... 11593
CDS Sequence 18668002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AATTGG)2
Repeat start & end within CDS Repeat start: 1151     Repeat end: 1162
Forward primer Primer sequence:   CACGCCTTTTGGAAAGAGCC     Tm(°C): 60.039     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCGAATTGTGCAAAGGCTT     Tm(°C): 59.324     GC (%): 45     Size: 20
Primer start, end within sequence and product size Start: 886     End: 1199     Product size (bp): 314
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Enzyme Id:  K02111
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668002     NCBI Gene Symbol: atpA
Gene Aliases CP95_p082
Gene description & Other designations Description:   ATP synthase CF1 alpha subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 10070 ...... 11593
CDS Sequence 18668002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ATCAT)2
Repeat start & end within CDS Repeat start: 1423     Repeat end: 1432
Forward primer Primer sequence:   CCCCTTTCGGTGGAAGAACA     Tm(°C): 59.89     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGCTTCGTCGGTAAATGTC     Tm(°C): 59.356     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1281     End: 1458     Product size (bp): 178
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Enzyme Id:  K02111
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668002     NCBI Gene Symbol: atpA
Gene Aliases CP95_p082
Gene description & Other designations Description:   ATP synthase CF1 alpha subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 10070 ...... 11593
CDS Sequence 18668002
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTGGTGACCGCTTTACAGGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGCTCTTTCCAAAAGGCGTG     Tm(°C): 60.039     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 624     End: 905     Product size (bp): 282
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