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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K02108

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668005     NCBI Gene Symbol: atpI
Gene Aliases CP95_p079
Gene description & Other designations Description:   ATP synthase CF0 subunit IV      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 14849 ...... 15592
CDS Sequence 18668005
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GCTTTA)2
Repeat start & end within CDS Repeat start: 415     Repeat end: 426
Forward primer Primer sequence:   AGCCGCACCCACAAATGATA     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCGCCTATATAAGCCGCAGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 380     End: 721     Product size (bp): 342
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Enzyme Id:  K02108

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668005     NCBI Gene Symbol: atpI
Gene Aliases CP95_p079
Gene description & Other designations Description:   ATP synthase CF0 subunit IV      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 14849 ...... 15592
CDS Sequence 18668005
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTT)3
Repeat start & end within CDS Repeat start: 601     Repeat end: 609
Forward primer Primer sequence:   AGCCGCACCCACAAATGATA     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCGCCTATATAAGCCGCAGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 380     End: 721     Product size (bp): 342
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Enzyme Id:  K02108

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668005     NCBI Gene Symbol: atpI
Gene Aliases CP95_p079
Gene description & Other designations Description:   ATP synthase CF0 subunit IV      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 14849 ...... 15592
CDS Sequence 18668005
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGCCGCACCCACAAATGATA     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGACCTTCCATGGATTCGCC     Tm(°C): 60.107     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 380     End: 736     Product size (bp): 357
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