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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K01963

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668040     NCBI Gene Symbol: accD
Gene Aliases CP95_p058
Gene description & Other designations Description:   acetyl-CoA carboxylase carboxyltransferase beta subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 58830 ...... 60305
CDS Sequence 18668040
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTAAAA)2
Repeat start & end within CDS Repeat start: 727     Repeat end: 738
Forward primer Primer sequence:   GGCATTTGTGGATTCAATGCG     Tm(°C): 59.071     GC (%): 47.619     Size: 21
Reverse primer Primer sequence:   CCTGTTTGAACGGCCTCAGT     Tm(°C): 60.535     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 673     End: 946     Product size (bp): 274
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Enzyme Id:  K01963

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668040     NCBI Gene Symbol: accD
Gene Aliases CP95_p058
Gene description & Other designations Description:   acetyl-CoA carboxylase carboxyltransferase beta subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 58830 ...... 60305
CDS Sequence 18668040
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GGA)3
Repeat start & end within CDS Repeat start: 873     Repeat end: 881
Forward primer Primer sequence:   ACATGGTCTCTCTGGATCCCA     Tm(°C): 59.991     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   TACTCGGCCAAACGTGTGAT     Tm(°C): 59.681     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 838     End: 1057     Product size (bp): 220
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Enzyme Id:  K01963

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668040     NCBI Gene Symbol: accD
Gene Aliases CP95_p058
Gene description & Other designations Description:   acetyl-CoA carboxylase carboxyltransferase beta subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 58830 ...... 60305
CDS Sequence 18668040
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ACAGGC)2
Repeat start & end within CDS Repeat start: 943     Repeat end: 954
Forward primer Primer sequence:   ACATGGTCTCTCTGGATCCCA     Tm(°C): 59.991     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   TACTCGGCCAAACGTGTGAT     Tm(°C): 59.681     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 838     End: 1057     Product size (bp): 220
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Enzyme Id:  K01963

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668040     NCBI Gene Symbol: accD
Gene Aliases CP95_p058
Gene description & Other designations Description:   acetyl-CoA carboxylase carboxyltransferase beta subunit      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 58830 ...... 60305
CDS Sequence 18668040
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GACAGCCTCGGTCCTTTTGA     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTAACGATCGTGGCGAGTA     Tm(°C): 59.973     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 75     End: 417     Product size (bp): 343
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