PlantPathMarks Database

Statistics

Number of enzymes: 1

Total Number of designed primers: 16

Number of PGTM primers: 3

Number of PMTM primers: 13

Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (CCCTC)2
Repeat start & end within CDS	Repeat start: 115 Repeat end: 124
Forward primer	Primer sequence: GATTCACTTCCCCTCTCCGC Tm(°C): 60.179 GC (%): 60 Size: 20
Reverse primer	Primer sequence: AGACTCGAGATCATCCCGCT Tm(°C): 60.179 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 36 End: 239 Product size (bp): 204
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (TTCAA)2
Repeat start & end within CDS	Repeat start: 463 Repeat end: 472
Forward primer	Primer sequence: TGGTCGGGTCTTGCAGTAAC Tm(°C): 59.966 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CACTTGGCTCAGGTGCAAAC Tm(°C): 59.969 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 384 End: 693 Product size (bp): 310
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (CAA)3
Repeat start & end within CDS	Repeat start: 542 Repeat end: 550
Forward primer	Primer sequence: TGGTCGGGTCTTGCAGTAAC Tm(°C): 59.966 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CACTTGGCTCAGGTGCAAAC Tm(°C): 59.969 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 384 End: 693 Product size (bp): 310
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Di Repeat sequence: (TA)4
Repeat start & end within CDS	Repeat start: 1327 Repeat end: 1334
Forward primer	Primer sequence: CAACCCCATGGCCCATCATA Tm(°C): 59.812 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AGACCTCTGCGCACAATACC Tm(°C): 60.108 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1148 End: 1468 Product size (bp): 321
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (GGATG)2
Repeat start & end within CDS	Repeat start: 1417 Repeat end: 1426
Forward primer	Primer sequence: CAACCCCATGGCCCATCATA Tm(°C): 59.812 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AGACCTCTGCGCACAATACC Tm(°C): 60.108 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1148 End: 1468 Product size (bp): 321
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (TTATC)2aca(AAG)3ctt(GATGAG)2
Repeat start & end within CDS	Repeat start: 1950 Repeat end: 1986
Forward primer	Primer sequence: TTCGGCAGGGGAGGAGATTA Tm(°C): 60.032 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCTTCCTCTCCAGCTGCAAA Tm(°C): 59.963 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1754 End: 2094 Product size (bp): 341
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (GATGT)2
Repeat start & end within CDS	Repeat start: 2105 Repeat end: 2114
Forward primer	Primer sequence: TTTGCAGCTGGAGAGGAAGG Tm(°C): 59.963 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TTGCTGCCTACCATCTGGAA Tm(°C): 59.009 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 2075 End: 2174 Product size (bp): 100
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108196302 NCBI Gene Symbol: LOC108196302
Gene Aliases	DCAR_025145
Gene description & Other designations	Description: glutamate--tRNA ligase; cytoplasmic Other designations: glutamate--tRNA ligase; cytoplasmic
Chromosome, Strand & Exon count	Chromosome: 7 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_030387.1 Gene Start and end within genomic accession: 24146001 ...... 24150117
CDS Sequence	108196302
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: TGGTCGGGTCTTGCAGTAAC Tm(°C): 59.966 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CACTTGGCTCAGGTGCAAAC Tm(°C): 59.969 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 384 End: 693 Product size (bp): 310
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (TGTTCG)2
Repeat start & end within CDS	Repeat start: 213 Repeat end: 224
Forward primer	Primer sequence: CGATGTCATCAATGCTGCCG Tm(°C): 60.042 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AAGAGAGCAGTTCTGGCACC Tm(°C): 59.964 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 88 End: 280 Product size (bp): 193
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (TTC)3
Repeat start & end within CDS	Repeat start: 317 Repeat end: 325
Forward primer	Primer sequence: GGTGCCAGAACTGCTCTCTT Tm(°C): 59.964 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCAATGCAGTCCAAGCCAAG Tm(°C): 59.754 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 261 End: 410 Product size (bp): 150
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (CTTGG)2
Repeat start & end within CDS	Repeat start: 392 Repeat end: 401
Forward primer	Primer sequence: GGTGCCAGAACTGCTCTCTT Tm(°C): 59.964 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CGGTGTATACTGGAGGCAGC Tm(°C): 60.249 GC (%): 60 Size: 20
Primer start, end within sequence and product size	Start: 261 End: 597 Product size (bp): 337
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (GTTGG)2
Repeat start & end within CDS	Repeat start: 1044 Repeat end: 1053
Forward primer	Primer sequence: CGACACGGTGCTACTTCAGT Tm(°C): 60.039 GC (%): 55 Size: 20
Reverse primer	Primer sequence: GTTCAACAGCCTCCTCGACA Tm(°C): 59.966 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 966 End: 1287 Product size (bp): 322
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (CAA)3
Repeat start & end within CDS	Repeat start: 1716 Repeat end: 1724
Forward primer	Primer sequence: CCTGGATGGCAGAATTGGGT Tm(°C): 60.033 GC (%): 55 Size: 20
Reverse primer	Primer sequence: GATATGATGGCCGGCTCCAA Tm(°C): 59.963 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1482 End: 1753 Product size (bp): 272
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108205115 NCBI Gene Symbol: LOC108205115
Gene Aliases	DCAR_001519
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial-like Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial-like
Chromosome, Strand & Exon count	Chromosome: 1 Strand: minus Exon count: 15
Gene Location within genomic sequence	Genomic accession No. NC_030381.1 Gene Start and end within genomic accession: 16820644 ...... 16834366
CDS Sequence	108205115
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: TGCCTCCAGTATACACCGGA Tm(°C): 60.033 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TTGGAACCCAAGTGCCTTGT Tm(°C): 60.033 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 580 End: 905 Product size (bp): 326
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108209786 NCBI Gene Symbol: LOC108209786
Gene Aliases	DCAR_006224
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial
Chromosome, Strand & Exon count	Chromosome: 2 Strand: plus Exon count: 14
Gene Location within genomic sequence	Genomic accession No. NC_030382.1 Gene Start and end within genomic accession: 23314190 ...... 23325002
CDS Sequence	108209786
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (TTGAT)2
Repeat start & end within CDS	Repeat start: 1322 Repeat end: 1331
Forward primer	Primer sequence: GGGGCTATTTGTCGAGGAGG Tm(°C): 59.893 GC (%): 60 Size: 20
Reverse primer	Primer sequence: TGCCCTTCCGCTTAACCAAT Tm(°C): 59.961 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 1277 End: 1554 Product size (bp): 278
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Enzyme Id: K01885
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 108209786 NCBI Gene Symbol: LOC108209786
Gene Aliases	DCAR_006224
Gene description & Other designations	Description: glutamate--tRNA ligase; chloroplastic/mitochondrial Other designations: glutamate--tRNA ligase; chloroplastic/mitochondrial
Chromosome, Strand & Exon count	Chromosome: 2 Strand: plus Exon count: 14
Gene Location within genomic sequence	Genomic accession No. NC_030382.1 Gene Start and end within genomic accession: 23314190 ...... 23325002
CDS Sequence	108209786
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: CACGGTCGTTTGTGCATTGT Tm(°C): 59.971 GC (%): 50 Size: 20
Reverse primer	Primer sequence: TGGAGATTGCCGGTAGGAGA Tm(°C): 60.033 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 109 End: 271 Product size (bp): 163
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