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Statistics

Number of enzymes: 1
Total Number of designed primers: 20
Number of PGTM primers:     5
Number of PMTM primers:     15
Number of Failed designed PMTM primers: 1

Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331615     NCBI Gene Symbol: LOC103331615
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13588637 ...... 13595992
CDS Sequence 103331615
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGCCT)2
Repeat start & end within CDS Repeat start: 87     Repeat end: 96
Forward primer Primer sequence:   TCCAGTGAGGACCAAAAGGC     Tm(°C): 59.89     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCTGTGCCGTACTCATCAGT     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 21     End: 226     Product size (bp): 206
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331615     NCBI Gene Symbol: LOC103331615
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13588637 ...... 13595992
CDS Sequence 103331615
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GTTCCA)2
Repeat start & end within CDS Repeat start: 790     Repeat end: 801
Forward primer Primer sequence:   ACATCAGTGGCTGGGTCTTG     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGGAGCATCAAACCACACAT     Tm(°C): 57.408     GC (%): 45     Size: 20
Primer start, end within sequence and product size Start: 684     End: 848     Product size (bp): 165
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331615     NCBI Gene Symbol: LOC103331615
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13588637 ...... 13595992
CDS Sequence 103331615
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAAAGCTGCTCAAGTTGGCG     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACCAGCTGATGTTGTGAGGG     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2195     End: 2363     Product size (bp): 169
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331646     NCBI Gene Symbol: LOC103331646
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13539069 ...... 13543623
CDS Sequence 103331646
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCCTT)2
Repeat start & end within CDS Repeat start: 88     Repeat end: 97
Forward primer Primer sequence:   CTGGCAAGCGAAACATCCTG     Tm(°C): 59.83     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGATGTGCGCCCAAATATG     Tm(°C): 60.04     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 58     End: 351     Product size (bp): 294
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331646     NCBI Gene Symbol: LOC103331646
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13539069 ...... 13543623
CDS Sequence 103331646
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GTTCCA)2
Repeat start & end within CDS Repeat start: 790     Repeat end: 801
Forward primer Primer sequence:   CAAGCGACAAATGCATGGCT     Tm(°C): 60.109     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCGGAGCATCAAACCACACA     Tm(°C): 59.892     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 720     End: 849     Product size (bp): 130
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331646     NCBI Gene Symbol: LOC103331646
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13539069 ...... 13543623
CDS Sequence 103331646
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCACG)2
Repeat start & end within CDS Repeat start: 2166     Repeat end: 2175
Forward primer Primer sequence:   GCAATCTGAAGCCAGCAACC     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCCACTTGCAAGCTCTCCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2095     End: 2300     Product size (bp): 206
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331646     NCBI Gene Symbol: LOC103331646
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 13539069 ...... 13543623
CDS Sequence 103331646
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTACATTTCCGGGGTTCGCA     Tm(°C): 59.964     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACCAGCTGATGTTGTGAGGG     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2221     End: 2363     Product size (bp): 143
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AAG)3
Repeat start & end within CDS Repeat start: 105     Repeat end: 113
Forward primer Primer sequence:   AAAGCCACCACCCATCTCAG     Tm(°C): 59.961     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCAAATGAGGAGGGGCATT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCC)3agtgacccttttgtcctca(CCACT)2
Repeat start & end within CDS Repeat start: 169     Repeat end: 206
Forward primer Primer sequence:   AAAGCCACCACCCATCTCAG     Tm(°C): 59.961     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCAAATGAGGAGGGGCATT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GTTTG)2
Repeat start & end within CDS Repeat start: 847     Repeat end: 856
Forward primer Primer sequence:   TGTGTTGCACGGAAAGAGGA     Tm(°C): 59.821     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CACCCTGATGAAACGGCTCT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 627     End: 934     Product size (bp): 308
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATT)2
Repeat start & end within CDS Repeat start: 1233     Repeat end: 1242
Forward primer Primer sequence:   AAATTCGGGTCTGATGCGGT     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTAGCACAGCCTCACAAGCT     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1122     End: 1422     Product size (bp): 301
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGTCA)2
Repeat start & end within CDS Repeat start: 1675     Repeat end: 1684
Forward primer Primer sequence:   CTACCTGGAGCGACACCAAG     Tm(°C): 60.109     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACCCGTTTGGCTTGAGGTAG     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1636     End: 1798     Product size (bp): 163
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCA)3gcgagaataaa(CTT)3
Repeat start & end within CDS Repeat start: 7     Repeat end: 35
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332629     NCBI Gene Symbol: LOC103332629
Gene Aliases
Gene description & Other designations Description:   methionine--tRNA ligase; chloroplastic/mitochondrial      Other designations:   methionine--tRNA ligase; chloroplastic/mitochondrial|probable methionine--tRNA ligase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 20491184 ...... 20495921
CDS Sequence 103332629
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAAGCCACCACCCATCTCAG     Tm(°C): 59.961     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCAAATGAGGAGGGGCATT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CGA)3
Repeat start & end within CDS Repeat start: 39     Repeat end: 47
Forward primer Primer sequence:   GGTCGCCACGTGATGATCTT     Tm(°C): 60.46     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACAGCCGATAATGTTGCCG     Tm(°C): 59.972     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 18     End: 207     Product size (bp): 190
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCCTT)2
Repeat start & end within CDS Repeat start: 157     Repeat end: 166
Forward primer Primer sequence:   TCACAATGACCCAGAAGGCC     Tm(°C): 59.962     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACAGCCGATAATGTTGCCG     Tm(°C): 59.972     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 91     End: 207     Product size (bp): 117
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GGTTCC)2
Repeat start & end within CDS Repeat start: 858     Repeat end: 869
Forward primer Primer sequence:   TGGAACTCCCTTCGCTCAAG     Tm(°C): 59.678     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCACCATTGCTCCCAATCA     Tm(°C): 59.297     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 706     End: 972     Product size (bp): 267
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ACCAC)2
Repeat start & end within CDS Repeat start: 2234     Repeat end: 2243
Forward primer Primer sequence:   GCAATCTGAAGCCAGCAACC     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGCCAACTTGAGCAGCTTTT     Tm(°C): 59.969     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2164     End: 2283     Product size (bp): 120
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AAG)3
Repeat start & end within CDS Repeat start: 2338     Repeat end: 2346
Forward primer Primer sequence:   AAAAGCTGCTCAAGTTGGCG     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TAGCAGGCCACTAGGTCAGT     Tm(°C): 59.959     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2264     End: 2401     Product size (bp): 138
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322759     NCBI Gene Symbol: LOC103322759
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 29491441 ...... 29499232
CDS Sequence 103322759
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCAACCTCGTTGTGCTGTTG     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCGATATGTGCGTCTCAGGA     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1565     End: 1704     Product size (bp): 140
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Enzyme Id:  K01874

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334288     NCBI Gene Symbol: LOC103334288
Gene Aliases
Gene description & Other designations Description:   probable methionine--tRNA ligase      Other designations:   probable methionine--tRNA ligase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 2408133 ...... 2411733
CDS Sequence 103334288
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCTCACCTTGGCACCATCAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCCCTCGTGCAGAGTCATA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 111     End: 529     Product size (bp): 419
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