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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K01845
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543967     NCBI Gene Symbol: GSA-AT
Gene Aliases GSA
Gene description & Other designations Description:   glutamate 1-semialdehyde 2;1-aminomutase      Other designations:   glutamate-1-semialdehyde 2;1-aminomutase; chloroplastic|glutamate-1-semialdehyde aminotransferase
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 2695554 ...... 2699300
CDS Sequence 543967
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TCT)6
Repeat start & end within CDS Repeat start: 103     Repeat end: 120
Forward primer Primer sequence:   CCATCTCACCGTCGATGCAA     Tm(°C): 60.46     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGCCAACTGACTTGAAAGC     Tm(°C): 60.04     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 78     End: 262     Product size (bp): 185
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Enzyme Id:  K01845
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543967     NCBI Gene Symbol: GSA-AT
Gene Aliases GSA
Gene description & Other designations Description:   glutamate 1-semialdehyde 2;1-aminomutase      Other designations:   glutamate-1-semialdehyde 2;1-aminomutase; chloroplastic|glutamate-1-semialdehyde aminotransferase
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 2695554 ...... 2699300
CDS Sequence 543967
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTT)3
Repeat start & end within CDS Repeat start: 1239     Repeat end: 1247
Forward primer Primer sequence:   GCTGGAAAGAAGACCGGTCA     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCCTCAAACTGTGATGGTGC     Tm(°C): 59.758     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1182     End: 1366     Product size (bp): 185
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Enzyme Id:  K01845
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543967     NCBI Gene Symbol: GSA-AT
Gene Aliases GSA
Gene description & Other designations Description:   glutamate 1-semialdehyde 2;1-aminomutase      Other designations:   glutamate-1-semialdehyde 2;1-aminomutase; chloroplastic|glutamate-1-semialdehyde aminotransferase
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 2695554 ...... 2699300
CDS Sequence 543967
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AACGAGTTTCGGTGCTCCAT     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTCCCGGAGAGTCAGGAAGA     Tm(°C): 60.034     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 416     End: 672     Product size (bp): 257
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