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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K01761

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337840     NCBI Gene Symbol: LOC103337840
Gene Aliases
Gene description & Other designations Description:   methionine gamma-lyase      Other designations:   methionine gamma-lyase
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 11254705 ...... 11257325
CDS Sequence 103337840
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AGAGC)2gcgatgacatggacaacgacgactacgtggccgccaaaaagtcca(T
GT)3cctcagct(GCGGCC)2
Repeat start & end within CDS Repeat start: 61     Repeat end: 144
Forward primer Primer sequence:   AACAACGCCACCTCCAAGAA     Tm(°C): 60.107     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTGAGCACCGTCGGATTGAA     Tm(°C): 59.966     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 39     End: 340     Product size (bp): 302
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Enzyme Id:  K01761

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337840     NCBI Gene Symbol: LOC103337840
Gene Aliases
Gene description & Other designations Description:   methionine gamma-lyase      Other designations:   methionine gamma-lyase
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 11254705 ...... 11257325
CDS Sequence 103337840
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ACG)3
Repeat start & end within CDS Repeat start: 529     Repeat end: 537
Forward primer Primer sequence:   TTCAATCCGACGGTGCTCAA     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTCGATCGCATTCCTCACCA     Tm(°C): 59.897     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 321     End: 581     Product size (bp): 261
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Enzyme Id:  K01761

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337840     NCBI Gene Symbol: LOC103337840
Gene Aliases
Gene description & Other designations Description:   methionine gamma-lyase      Other designations:   methionine gamma-lyase
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 11254705 ...... 11257325
CDS Sequence 103337840
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGTGT)2
Repeat start & end within CDS Repeat start: 802     Repeat end: 811
Forward primer Primer sequence:   GTTTATTAGCGGCGGTGCTG     Tm(°C): 59.972     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCATGGACTTGAGCAGCTCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 770     End: 1056     Product size (bp): 287
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Enzyme Id:  K01761

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337840     NCBI Gene Symbol: LOC103337840
Gene Aliases
Gene description & Other designations Description:   methionine gamma-lyase      Other designations:   methionine gamma-lyase
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 11254705 ...... 11257325
CDS Sequence 103337840
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTTTATTAGCGGCGGTGCTG     Tm(°C): 59.972     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGAACTGGGTGCAGTTTTG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 770     End: 1159     Product size (bp): 390
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