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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3
Number of Failed designed PMTM primers: 1

Enzyme Id:  K01760

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339313     NCBI Gene Symbol: LOC103339313
Gene Aliases
Gene description & Other designations Description:   cystathionine beta-lyase; chloroplastic      Other designations:   cystathionine beta-lyase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   13
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 5415636 ...... 5422317
CDS Sequence 103339313
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTT)3
Repeat start & end within CDS Repeat start: 159     Repeat end: 167
Forward primer Primer sequence:   ACAACGCTGAGGGGAAAGAG     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TAAGCAGTCCGCAACACCAT     Tm(°C): 59.964     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 138     End: 257     Product size (bp): 120
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Enzyme Id:  K01760

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339313     NCBI Gene Symbol: LOC103339313
Gene Aliases
Gene description & Other designations Description:   cystathionine beta-lyase; chloroplastic      Other designations:   cystathionine beta-lyase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   13
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 5415636 ...... 5422317
CDS Sequence 103339313
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATG)2
Repeat start & end within CDS Repeat start: 330     Repeat end: 339
Forward primer Primer sequence:   ATGGTGTTGCGGACTGCTTA     Tm(°C): 59.964     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CGGCAACAATCTCCTCACCT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 238     End: 555     Product size (bp): 318
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Enzyme Id:  K01760

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339313     NCBI Gene Symbol: LOC103339313
Gene Aliases
Gene description & Other designations Description:   cystathionine beta-lyase; chloroplastic      Other designations:   cystathionine beta-lyase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   13
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 5415636 ...... 5422317
CDS Sequence 103339313
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCTGC)2
Repeat start & end within CDS Repeat start: 1269     Repeat end: 1278
Forward primer Primer sequence:   CCCATCCACGAGTGACACAA     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCAGATGGTATGCTTGCGTG     Tm(°C): 59.971     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1069     End: 1306     Product size (bp): 238
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Enzyme Id:  K01760

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339313     NCBI Gene Symbol: LOC103339313
Gene Aliases
Gene description & Other designations Description:   cystathionine beta-lyase; chloroplastic      Other designations:   cystathionine beta-lyase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   13
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 5415636 ...... 5422317
CDS Sequence 103339313
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTT)3(TC)4*
Repeat start & end within CDS Repeat start: 8     Repeat end: 23
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K01760

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339313     NCBI Gene Symbol: LOC103339313
Gene Aliases
Gene description & Other designations Description:   cystathionine beta-lyase; chloroplastic      Other designations:   cystathionine beta-lyase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   13
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 5415636 ...... 5422317
CDS Sequence 103339313
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGCCACTGGATCTAGGAGCA     Tm(°C): 60.032     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCAGATGGTATGCTTGCGTG     Tm(°C): 59.971     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 814     End: 1306     Product size (bp): 493
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