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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 1

Enzyme Id:  K01749

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108219287     NCBI Gene Symbol: LOC108219287
Gene Aliases DCAR_013737
Gene description & Other designations Description:   porphobilinogen deaminase; chloroplastic      Other designations:   porphobilinogen deaminase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_030384.1      Gene Start and end within genomic accession: 27777044 ...... 27782335
CDS Sequence 108219287
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ATGAT)2
Repeat start & end within CDS Repeat start: 1061     Repeat end: 1070
Forward primer Primer sequence:   TGCTGGATACGCTTCCAAGG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCAGCATCCTTCCCCATTG     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 947     End: 1091     Product size (bp): 145
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Enzyme Id:  K01749

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108219287     NCBI Gene Symbol: LOC108219287
Gene Aliases DCAR_013737
Gene description & Other designations Description:   porphobilinogen deaminase; chloroplastic      Other designations:   porphobilinogen deaminase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_030384.1      Gene Start and end within genomic accession: 27777044 ...... 27782335
CDS Sequence 108219287
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CCG)3
Repeat start & end within CDS Repeat start: 41     Repeat end: 49
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K01749

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108219287     NCBI Gene Symbol: LOC108219287
Gene Aliases DCAR_013737
Gene description & Other designations Description:   porphobilinogen deaminase; chloroplastic      Other designations:   porphobilinogen deaminase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_030384.1      Gene Start and end within genomic accession: 27777044 ...... 27782335
CDS Sequence 108219287
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AATGACGGTGTTGTGCAAGC     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACCAGCATCCTTCCCCATTG     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 687     End: 1091     Product size (bp): 405
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