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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K01739

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330036     NCBI Gene Symbol: LOC103330036
Gene Aliases
Gene description & Other designations Description:   cystathionine gamma-synthase 1; chloroplastic-like      Other designations:   LOW QUALITY PROTEIN: cystathionine gamma-synthase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 22099770 ...... 22106534
CDS Sequence 103330036
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CGGGT)2cgacgccacggcgccgtttcacgg(CCTCT)2
Repeat start & end within CDS Repeat start: 68     Repeat end: 111
Forward primer Primer sequence:   TTTCACCTCTTCCCAACCCG     Tm(°C): 59.89     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGGAATGCCTGAGTTGGAGT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 29     End: 242     Product size (bp): 214
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Enzyme Id:  K01739

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330036     NCBI Gene Symbol: LOC103330036
Gene Aliases
Gene description & Other designations Description:   cystathionine gamma-synthase 1; chloroplastic-like      Other designations:   LOW QUALITY PROTEIN: cystathionine gamma-synthase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 22099770 ...... 22106534
CDS Sequence 103330036
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AAC)3(CAACTC)2*
Repeat start & end within CDS Repeat start: 217     Repeat end: 233
Forward primer Primer sequence:   TTTCCTCCCAACTTCGTCCG     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCAGTAACTATGCCGCGACC     Tm(°C): 59.898     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 123     End: 451     Product size (bp): 329
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Enzyme Id:  K01739

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330036     NCBI Gene Symbol: LOC103330036
Gene Aliases
Gene description & Other designations Description:   cystathionine gamma-synthase 1; chloroplastic-like      Other designations:   LOW QUALITY PROTEIN: cystathionine gamma-synthase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 22099770 ...... 22106534
CDS Sequence 103330036
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GCCCTT)2
Repeat start & end within CDS Repeat start: 967     Repeat end: 978
Forward primer Primer sequence:   GAAAGCCCTGGAGTCTGCAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCGGCAAGGACATCATTGT     Tm(°C): 59.962     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 794     End: 1043     Product size (bp): 250
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Enzyme Id:  K01739

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330036     NCBI Gene Symbol: LOC103330036
Gene Aliases
Gene description & Other designations Description:   cystathionine gamma-synthase 1; chloroplastic-like      Other designations:   LOW QUALITY PROTEIN: cystathionine gamma-synthase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 22099770 ...... 22106534
CDS Sequence 103330036
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCCTGATTTGAAGGAGGCCT     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGCAGACTCCAGGGCTTTC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 362     End: 813     Product size (bp): 452
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