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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K01634
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322098     NCBI Gene Symbol: LOC103322098
Gene Aliases
Gene description & Other designations Description:   sphingosine-1-phosphate lyase      Other designations:   sphingosine-1-phosphate lyase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 21038903 ...... 21044366
CDS Sequence 103322098
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCTGGT)2
Repeat start & end within CDS Repeat start: 75     Repeat end: 86
Forward primer Primer sequence:   TCTTGTCCGAATACGAGCCT     Tm(°C): 58.529     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GTTCTCGTGCAGGACTTGGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 55     End: 155     Product size (bp): 101
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Enzyme Id:  K01634
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322098     NCBI Gene Symbol: LOC103322098
Gene Aliases
Gene description & Other designations Description:   sphingosine-1-phosphate lyase      Other designations:   sphingosine-1-phosphate lyase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 21038903 ...... 21044366
CDS Sequence 103322098
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGGGAG)2tcatagagcaaatgaaagatg(AGAAA)2
Repeat start & end within CDS Repeat start: 320     Repeat end: 362
Forward primer Primer sequence:   GTCCGAATACGAGCCTCTGG     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CATTTCCCTTGCCAAGCCAC     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 59     End: 385     Product size (bp): 327
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Enzyme Id:  K01634
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322098     NCBI Gene Symbol: LOC103322098
Gene Aliases
Gene description & Other designations Description:   sphingosine-1-phosphate lyase      Other designations:   sphingosine-1-phosphate lyase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 21038903 ...... 21044366
CDS Sequence 103322098
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTCCGAATACGAGCCTCTGG     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CAGAGTGGCTTTGAGTCCGT     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 59     End: 173     Product size (bp): 115
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