image


Statistics

Number of enzymes: 1
Total Number of designed primers: 18
Number of PGTM primers:     3
Number of PMTM primers:     15

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAACT)2
Repeat start & end within CDS Repeat start: 1073     Repeat end: 1082
Forward primer Primer sequence:   CTTGAAGCAACAGGGTGGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGTCCTGACCACCCTGATGA     Tm(°C): 59.883     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1046     End: 1182     Product size (bp): 137
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTT)3
Repeat start & end within CDS Repeat start: 1441     Repeat end: 1449
Forward primer Primer sequence:   AGGGTCTGCCTCTCGTAGTT     Tm(°C): 59.96     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCAGCAAGTCGACCGTCAG     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1250     End: 1583     Product size (bp): 334
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTATG)2aatctttgcaatcatgtggatctggagtgctagctgacggtcgactt
g(CTGAT)2
Repeat start & end within CDS Repeat start: 1522     Repeat end: 1589
Forward primer Primer sequence:   CCCCTCAGAGACCTGGAGTT     Tm(°C): 60.252     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GTCTACCAGATTCCTGACGCA     Tm(°C): 59.523     GC (%): 52.381     Size: 21
Primer start, end within sequence and product size Start: 1354     End: 1653     Product size (bp): 300
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GAA)3(AAGAA)2*
Repeat start & end within CDS Repeat start: 1714     Repeat end: 1727
Forward primer Primer sequence:   ACGGTCGACTTGCTGATCTG     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GACATCACTTGCACGTGACG     Tm(°C): 59.838     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1567     End: 1898     Product size (bp): 332
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ATTCTG)2
Repeat start & end within CDS Repeat start: 2117     Repeat end: 2128
Forward primer Primer sequence:   ATTGGAAGGCCATGTCCAGG     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGCATGCAGCCACAACATC     Tm(°C): 60.038     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1950     End: 2200     Product size (bp): 251
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTTGG)2(TTGGAG)2*
Repeat start & end within CDS Repeat start: 2687     Repeat end: 2704
Forward primer Primer sequence:   TTGCATGGACCCAAACCAGA     Tm(°C): 59.813     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GATAGCTTCTCATGCCCGCT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2653     End: 2953     Product size (bp): 301
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341411     NCBI Gene Symbol: LOC103341411
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 4      Other designations:   phosphoenolpyruvate carboxylase 4
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341411
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATTGGAAGGCCATGTCCAGG     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTCCTTGCTCAGTTGACCGT     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1950     End: 2337     Product size (bp): 388
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGATG)2
Repeat start & end within CDS Repeat start: 699     Repeat end: 708
Forward primer Primer sequence:   AAGGACTCCTCCAACCCCTC     Tm(°C): 60.548     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GTCCCCACCCATCCAAGAAG     Tm(°C): 60.034     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 677     End: 860     Product size (bp): 184
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 1324     Repeat end: 1331
Forward primer Primer sequence:   TGTGTTACCGGTCACTCTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCAATCTCCAGGTGCCTTGT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1234     End: 1408     Product size (bp): 175
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTG)3
Repeat start & end within CDS Repeat start: 1706     Repeat end: 1714
Forward primer Primer sequence:   TGCTGATCTTGAGGCTGCTC     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGAATTGTGTCGGGTGGCT     Tm(°C): 59.962     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1685     End: 1976     Product size (bp): 292
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAG)3
Repeat start & end within CDS Repeat start: 1922     Repeat end: 1930
Forward primer Primer sequence:   TGGTCGAGGTGGAACAGTTG     Tm(°C): 59.894     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCGTGCTCAAGTGTAGCTG     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1898     End: 2087     Product size (bp): 190
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 2552     Repeat end: 2559
Forward primer Primer sequence:   TGGGTTTGGGGCAGCATTTA     Tm(°C): 60.179     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAACCTGGAGGACAAGGCTC     Tm(°C): 60.322     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 2357     End: 2604     Product size (bp): 248
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328478     NCBI Gene Symbol: LOC103328478
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase 2      Other designations:   phosphoenolpyruvate carboxylase 2
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 10352526 ...... 10358701
CDS Sequence 103328478
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTTCTTGGATGGGTGGGGAC     Tm(°C): 60.034     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCAGAGTGACCGGTAACACA     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 841     End: 1253     Product size (bp): 413
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326102     NCBI Gene Symbol: LOC103326102
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase; housekeeping isozyme      Other designations:   phosphoenolpyruvate carboxylase; housekeeping isozyme
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 11407433 ...... 11414160
CDS Sequence 103326102
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTG)3
Repeat start & end within CDS Repeat start: 275     Repeat end: 283
Forward primer Primer sequence:   CTGGAGAAGTTGGCGTCCAT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCTTGTTTCGTCTGCGGTA     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 15     End: 361     Product size (bp): 347
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326102     NCBI Gene Symbol: LOC103326102
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase; housekeeping isozyme      Other designations:   phosphoenolpyruvate carboxylase; housekeeping isozyme
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 11407433 ...... 11414160
CDS Sequence 103326102
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)4
Repeat start & end within CDS Repeat start: 638     Repeat end: 645
Forward primer Primer sequence:   CTGGTCCTCACTGCTCATCC     Tm(°C): 59.822     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGGGGCATTATGGGGAACAC     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 498     End: 827     Product size (bp): 330
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326102     NCBI Gene Symbol: LOC103326102
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase; housekeeping isozyme      Other designations:   phosphoenolpyruvate carboxylase; housekeeping isozyme
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 11407433 ...... 11414160
CDS Sequence 103326102
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCCGTG)2
Repeat start & end within CDS Repeat start: 1001     Repeat end: 1012
Forward primer Primer sequence:   GTCTATGTGGCGCTGCAATG     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGAATGTCAGAGTGCCCACA     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 974     End: 1183     Product size (bp): 210
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326102     NCBI Gene Symbol: LOC103326102
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase; housekeeping isozyme      Other designations:   phosphoenolpyruvate carboxylase; housekeeping isozyme
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 11407433 ...... 11414160
CDS Sequence 103326102
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGCTC)2
Repeat start & end within CDS Repeat start: 1716     Repeat end: 1725
Forward primer Primer sequence:   TGAGCTCCTGCAACGTGAAT     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CACCTCCCCTTCCAACAGTC     Tm(°C): 59.963     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1619     End: 1929     Product size (bp): 311
JBrowse View      JBrowse

Enzyme Id:  K01595

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326102     NCBI Gene Symbol: LOC103326102
Gene Aliases
Gene description & Other designations Description:   phosphoenolpyruvate carboxylase; housekeeping isozyme      Other designations:   phosphoenolpyruvate carboxylase; housekeeping isozyme
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   11
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 11407433 ...... 11414160
CDS Sequence 103326102
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGTTCCCCATAATGCCCCAC     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGGAGTTCATCATTGCAGCG     Tm(°C): 59.973     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 809     End: 1003     Product size (bp): 195
JBrowse View      JBrowse