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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2
Number of Failed designed PMTM primers: 1

Enzyme Id:  K01006

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322886     NCBI Gene Symbol: LOC103322886
Gene Aliases
Gene description & Other designations Description:   pyruvate; phosphate dikinase; chloroplastic      Other designations:   pyruvate; phosphate dikinase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   22
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 30620307 ...... 30627199
CDS Sequence 103322886
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CAAACC)2
Repeat start & end within CDS Repeat start: 197     Repeat end: 208
Forward primer Primer sequence:   AGGCATGCTCATAAGGACCG     Tm(°C): 59.893     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTGCCCTGGCTCATACTTGT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 17     End: 230     Product size (bp): 214
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Enzyme Id:  K01006

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322886     NCBI Gene Symbol: LOC103322886
Gene Aliases
Gene description & Other designations Description:   pyruvate; phosphate dikinase; chloroplastic      Other designations:   pyruvate; phosphate dikinase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   22
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 30620307 ...... 30627199
CDS Sequence 103322886
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ACAAAG)2
Repeat start & end within CDS Repeat start: 1481     Repeat end: 1492
Forward primer Primer sequence:   AACCACAGCACCTTGACCAA     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGTCAAGATCCCAGCAGCTG     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1426     End: 1661     Product size (bp): 236
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Enzyme Id:  K01006

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322886     NCBI Gene Symbol: LOC103322886
Gene Aliases
Gene description & Other designations Description:   pyruvate; phosphate dikinase; chloroplastic      Other designations:   pyruvate; phosphate dikinase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   22
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 30620307 ...... 30627199
CDS Sequence 103322886
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AGACT)2atgtttcgtgttctccattcagggtacctatt(GCTAG)2(AGC)3*
Repeat start & end within CDS Repeat start: 2869     Repeat end: 2927
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K01006

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322886     NCBI Gene Symbol: LOC103322886
Gene Aliases
Gene description & Other designations Description:   pyruvate; phosphate dikinase; chloroplastic      Other designations:   pyruvate; phosphate dikinase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   22
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 30620307 ...... 30627199
CDS Sequence 103322886
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAAAGGTGCAAACCTGGCAG     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCACTCTTTGCAGCCAAACC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 389     End: 697     Product size (bp): 309
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