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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K00801
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335730     NCBI Gene Symbol: LOC103335730
Gene Aliases
Gene description & Other designations Description:   squalene synthase-like      Other designations:   squalene synthase-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11910503 ...... 11918201
CDS Sequence 103335730
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AT)4
Repeat start & end within CDS Repeat start: 299     Repeat end: 306
Forward primer Primer sequence:   AGCTTCGTAACGCTGTCTGT     Tm(°C): 59.686     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AAGCCCTGCTACATAGTGGC     Tm(°C): 59.819     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 181     End: 524     Product size (bp): 344
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Enzyme Id:  K00801
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335730     NCBI Gene Symbol: LOC103335730
Gene Aliases
Gene description & Other designations Description:   squalene synthase-like      Other designations:   squalene synthase-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11910503 ...... 11918201
CDS Sequence 103335730
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (TC)4
Repeat start & end within CDS Repeat start: 586     Repeat end: 593
Forward primer Primer sequence:   GTCCAAGCTTTTCCACGCTG     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAGCATTGCACAGCCTTGT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 539     End: 767     Product size (bp): 229
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Enzyme Id:  K00801
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335730     NCBI Gene Symbol: LOC103335730
Gene Aliases
Gene description & Other designations Description:   squalene synthase-like      Other designations:   squalene synthase-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11910503 ...... 11918201
CDS Sequence 103335730
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ACA)3
Repeat start & end within CDS Repeat start: 902     Repeat end: 910
Forward primer Primer sequence:   ACAAGGCTGTGCAATGCTTG     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTTTGCTGTAAGGCCCCTCC     Tm(°C): 60.252     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 748     End: 959     Product size (bp): 212
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Enzyme Id:  K00801
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335730     NCBI Gene Symbol: LOC103335730
Gene Aliases
Gene description & Other designations Description:   squalene synthase-like      Other designations:   squalene synthase-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11910503 ...... 11918201
CDS Sequence 103335730
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGGCCTCGGATTCTCTCTCA     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAGCATTGCACAGCCTTGT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 574     End: 767     Product size (bp): 194
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