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Statistics

Number of enzymes: 1
Total Number of designed primers: 26
Number of PGTM primers:     4
Number of PMTM primers:     22

Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTC)3
Repeat start & end within CDS Repeat start: 42     Repeat end: 50
Forward primer Primer sequence:   TCGAAGACCACCATTTCCTTC     Tm(°C): 57.946     GC (%): 47.619     Size: 21
Reverse primer Primer sequence:   TAGCCTCAGCGACTTCCTCT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 21     End: 354     Product size (bp): 334
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCCAA)2
Repeat start & end within CDS Repeat start: 143     Repeat end: 152
Forward primer Primer sequence:   CCTTCCTTCTCCACCGCATT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TAGCCTCAGCGACTTCCTCT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 90     End: 354     Product size (bp): 265
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TCG)3
Repeat start & end within CDS Repeat start: 272     Repeat end: 280
Forward primer Primer sequence:   CCTTCCTTCTCCACCGCATT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TAGCCTCAGCGACTTCCTCT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 90     End: 354     Product size (bp): 265
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AAAGCC)2aaatccgcttcatcagattcccctg(CTT)3ccccggctgctgaag
ctgttacgccca(CTC)3
Repeat start & end within CDS Repeat start: 355     Repeat end: 436
Forward primer Primer sequence:   AGAGGAAGTCGCTGAGGCTA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTGGCCACAGTCTTTTTGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 335     End: 515     Product size (bp): 181
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCC)3ggaatt(GCCCA)2
Repeat start & end within CDS Repeat start: 613     Repeat end: 637
Forward primer Primer sequence:   CCAAAAAGACTGTGGCCACG     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGAATGTTGGCACCGAGAGA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 496     End: 810     Product size (bp): 315
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCT)3
Repeat start & end within CDS Repeat start: 904     Repeat end: 912
Forward primer Primer sequence:   TCTCTCGGTGCCAACATTCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCACCATTGATTGCCACAGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 791     End: 1018     Product size (bp): 228
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ATTTGT)2
Repeat start & end within CDS Repeat start: 1058     Repeat end: 1069
Forward primer Primer sequence:   TCTCTCGGTGCCAACATTCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCATGGGGCTGAAGTTGCTT     Tm(°C): 59.887     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 791     End: 1129     Product size (bp): 339
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103341467     NCBI Gene Symbol: LOC103341467
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103341467
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGAGGAAGTCGCTGAGGCTA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTGGCCACAGTCTTTTTGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 335     End: 515     Product size (bp): 181
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 45     Repeat end: 52
Forward primer Primer sequence:   CGACACCCAGTGATCTCTCG     Tm(°C): 59.9     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GGACAAAGTTGCAGGCCTTG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 18     End: 161     Product size (bp): 144
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 306     Repeat end: 314
Forward primer Primer sequence:   CAAGGCCTGCAACTTTGTCC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTGTCCCACAGGCACATCTT     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 142     End: 455     Product size (bp): 314
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AGATA)2gaagtgg(GTGAT)2atgtgagatagagacagacaaagcaactg(TT
GAAT)2
Repeat start & end within CDS Repeat start: 702     Repeat end: 769
Forward primer Primer sequence:   ATCCCCAACAATGAGCCAGG     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAATTGCTTGGCCAACAGCT     Tm(°C): 59.966     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 650     End: 843     Product size (bp): 194
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGAT)2
Repeat start & end within CDS Repeat start: 1017     Repeat end: 1026
Forward primer Primer sequence:   AGCTGTTGGCCAAGCAATTG     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GCAGTGTACCATGAGCACCT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 824     End: 1065     Product size (bp): 242
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAAGA)2
Repeat start & end within CDS Repeat start: 1126     Repeat end: 1135
Forward primer Primer sequence:   AGCTGTTGGCCAAGCAATTG     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GGATGCACCTGAGGAGATGG     Tm(°C): 59.892     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 824     End: 1159     Product size (bp): 336
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAAAA)2
Repeat start & end within CDS Repeat start: 1274     Repeat end: 1283
Forward primer Primer sequence:   CCATCTCCTCAGGTGCATCC     Tm(°C): 59.892     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCATCCCAGTACGCATTTGC     Tm(°C): 60.249     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1140     End: 1450     Product size (bp): 311
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AGCTGT)2
Repeat start & end within CDS Repeat start: 1398     Repeat end: 1409
Forward primer Primer sequence:   CCATCTCCTCAGGTGCATCC     Tm(°C): 59.892     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCATCCCAGTACGCATTTGC     Tm(°C): 60.249     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1140     End: 1450     Product size (bp): 311
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103332293     NCBI Gene Symbol: LOC103332293
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 1 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   23
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 18355840 ...... 18364390
CDS Sequence 103332293
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCTCGCCTTCTCTCCTCATC     Tm(°C): 59.968     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GGACAAAGTTGCAGGCCTTG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 57     End: 161     Product size (bp): 105
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336058     NCBI Gene Symbol: LOC103336058
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic|dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 15488164 ...... 15493430
CDS Sequence 103336058
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GGC)3
Repeat start & end within CDS Repeat start: 471     Repeat end: 479
Forward primer Primer sequence:   GGATAATGTAGGTCCCGCGG     Tm(°C): 60.038     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AGCCACAATCCTCCCCAATG     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 440     End: 623     Product size (bp): 184
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336058     NCBI Gene Symbol: LOC103336058
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic|dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 15488164 ...... 15493430
CDS Sequence 103336058
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GCAGTG)2
Repeat start & end within CDS Repeat start: 979     Repeat end: 990
Forward primer Primer sequence:   CTGTCGTCAACTCTGGCTGT     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCTGCATCCTGAAGCACTGG     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 916     End: 1033     Product size (bp): 118
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336058     NCBI Gene Symbol: LOC103336058
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic|dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 15488164 ...... 15493430
CDS Sequence 103336058
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AATAC)2
Repeat start & end within CDS Repeat start: 1115     Repeat end: 1124
Forward primer Primer sequence:   CTGTCGTCAACTCTGGCTGT     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCAGTTCCAGGTGGCAGAAT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 916     End: 1198     Product size (bp): 283
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336058     NCBI Gene Symbol: LOC103336058
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic      Other designations:   LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex; chloroplastic|dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 15488164 ...... 15493430
CDS Sequence 103336058
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CATTGGGGAGGATTGTGGCT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACAGCCAGAGTTGACGACAG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 604     End: 935     Product size (bp): 332
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGTGA)2
Repeat start & end within CDS Repeat start: 553     Repeat end: 562
Forward primer Primer sequence:   TTTCATCCGATGCAGCCCTT     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GTAGGCTCAGGTGACGCTTT     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 319     End: 667     Product size (bp): 349
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CATCAG)2
Repeat start & end within CDS Repeat start: 617     Repeat end: 628
Forward primer Primer sequence:   TTTCATCCGATGCAGCCCTT     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GTAGGCTCAGGTGACGCTTT     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 319     End: 667     Product size (bp): 349
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCATTA)2gattacaccgatatccctcattctcagatacggaagatcacagctt
cgag(ATT)3atcaaagcaaacaattcctc(ATT)3
Repeat start & end within CDS Repeat start: 925     Repeat end: 1024
Forward primer Primer sequence:   GAACAGGTCCTGATGGAAGCA     Tm(°C): 59.996     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   GCTTCCCACCTGATGCTTCT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 826     End: 1104     Product size (bp): 279
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCT)3
Repeat start & end within CDS Repeat start: 1135     Repeat end: 1143
Forward primer Primer sequence:   AGAAGCATCAGGTGGGAAGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCTGACCTCCTCAGCTAT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1085     End: 1315     Product size (bp): 231
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGCAG)2
Repeat start & end within CDS Repeat start: 1227     Repeat end: 1236
Forward primer Primer sequence:   AGAAGCATCAGGTGGGAAGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCTGACCTCCTCAGCTAT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1085     End: 1315     Product size (bp): 231
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Enzyme Id:  K00627
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327668     NCBI Gene Symbol: LOC103327668
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial      Other designations:   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3161638 ...... 3168042
CDS Sequence 103327668
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGAAGCATCAGGTGGGAAGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCTGACCTCCTCAGCTAT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1085     End: 1315     Product size (bp): 231
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