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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     2
Number of PMTM primers:     2
Number of Failed designed PMTM primers: 1

Enzyme Id:  K00626

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329265     NCBI Gene Symbol: LOC103329265
Gene Aliases
Gene description & Other designations Description:   acetyl-CoA acetyltransferase; cytosolic 1      Other designations:   acetyl-CoA acetyltransferase; cytosolic 1
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 17184939 ...... 17189522
CDS Sequence 103329265
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TGT)3
Repeat start & end within CDS Repeat start: 366     Repeat end: 374
Forward primer Primer sequence:   CACAAAGCATCCAGTTGGGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGAGATCCCTTCCTCGCTTC     Tm(°C): 59.969     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 337     End: 439     Product size (bp): 103
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Enzyme Id:  K00626

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329265     NCBI Gene Symbol: LOC103329265
Gene Aliases
Gene description & Other designations Description:   acetyl-CoA acetyltransferase; cytosolic 1      Other designations:   acetyl-CoA acetyltransferase; cytosolic 1
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 17184939 ...... 17189522
CDS Sequence 103329265
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CTGCTC)2
Repeat start & end within CDS Repeat start: 917     Repeat end: 928
Forward primer Primer sequence:   TACTGCTGGCAATGCTTCCA     Tm(°C): 59.962     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CCAATGCTACAGCTCCACCA     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 770     End: 1086     Product size (bp): 317
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Enzyme Id:  K00626

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103329265     NCBI Gene Symbol: LOC103329265
Gene Aliases
Gene description & Other designations Description:   acetyl-CoA acetyltransferase; cytosolic 1      Other designations:   acetyl-CoA acetyltransferase; cytosolic 1
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 17184939 ...... 17189522
CDS Sequence 103329265
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ACAGCAGAAGCATGTTCCGA     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CGAGATCCCTTCCTCGCTTC     Tm(°C): 59.969     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 12     End: 439     Product size (bp): 428
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Enzyme Id:  K00626

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324371     NCBI Gene Symbol: LOC103324371
Gene Aliases
Gene description & Other designations Description:   acetyl-CoA acetyltransferase; cytosolic 1      Other designations:   acetyl-CoA acetyltransferase; cytosolic 1
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 123074 ...... 126876
CDS Sequence 103324371
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TCT)3
Repeat start & end within CDS Repeat start: 13     Repeat end: 21
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K00626

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324371     NCBI Gene Symbol: LOC103324371
Gene Aliases
Gene description & Other designations Description:   acetyl-CoA acetyltransferase; cytosolic 1      Other designations:   acetyl-CoA acetyltransferase; cytosolic 1
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 123074 ...... 126876
CDS Sequence 103324371
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTCCAGCCCTTGCAATACCA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGAACAAGGGCAGATGCTCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 910     End: 1204     Product size (bp): 295
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