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Statistics

Number of enzymes: 1
Total Number of designed primers: 14
Number of PGTM primers:     2
Number of PMTM primers:     12

Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AG)4ctcaagtttgccttggaatctttc(TGGGA)2
Repeat start & end within CDS Repeat start: 158     Repeat end: 199
Forward primer Primer sequence:   CGTCCCACTCTTCTTCGCTT     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TAAGACCTGCAGCCACGTTT     Tm(°C): 59.892     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 63     End: 240     Product size (bp): 178
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGGA)2
Repeat start & end within CDS Repeat start: 557     Repeat end: 566
Forward primer Primer sequence:   GGGAATGCACATGTTCCTGC     Tm(°C): 59.826     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGTGGGCTCATCAAACTGGA     Tm(°C): 59.962     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 414     End: 743     Product size (bp): 330
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCT)3
Repeat start & end within CDS Repeat start: 1318     Repeat end: 1326
Forward primer Primer sequence:   GTGACGAATGAGGCTGTCCA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATTCCTCTCGGGCTCTCCAT     Tm(°C): 60.105     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1293     End: 1598     Product size (bp): 306
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ACC)3
Repeat start & end within CDS Repeat start: 1417     Repeat end: 1425
Forward primer Primer sequence:   AATGAGGCTGTCCAGAAGGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATTCCTCTCGGGCTCTCCAT     Tm(°C): 60.105     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1299     End: 1598     Product size (bp): 300
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CAATG)2(GGA)3*
Repeat start & end within CDS Repeat start: 1509     Repeat end: 1526
Forward primer Primer sequence:   AATGAGGCTGTCCAGAAGGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATTCCTCTCGGGCTCTCCAT     Tm(°C): 60.105     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1299     End: 1598     Product size (bp): 300
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TGC)3
Repeat start & end within CDS Repeat start: 2370     Repeat end: 2378
Forward primer Primer sequence:   TCCTCCCTGAGGGACTGAAG     Tm(°C): 59.958     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCCTCAACATCTGCCGATTG     Tm(°C): 59.62     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2161     End: 2403     Product size (bp): 243
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103330179     NCBI Gene Symbol: LOC103330179
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 11756876 ...... 11762168
CDS Sequence 103330179
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGTTGTCTCCACATCCTGCT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCCTTCTGGACAGCCTCATT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1082     End: 1318     Product size (bp): 237
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AG)4ctcaagtttgctttggaatctttc(TGGGA)2
Repeat start & end within CDS Repeat start: 41     Repeat end: 82
Forward primer Primer sequence:   TGGCGTCTCACATTGTTGGA     Tm(°C): 59.892     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTGCTGGGGATGTGCTTGAT     Tm(°C): 59.96     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1     End: 178     Product size (bp): 178
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGAT)2
Repeat start & end within CDS Repeat start: 824     Repeat end: 833
Forward primer Primer sequence:   AGCCCACGCTTGTTATGGAT     Tm(°C): 59.744     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCCATCAACCAATCCGGCAA     Tm(°C): 59.961     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 619     End: 884     Product size (bp): 266
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCT)3
Repeat start & end within CDS Repeat start: 1201     Repeat end: 1209
Forward primer Primer sequence:   GACCAAGCTGGACCAGGAAA     Tm(°C): 59.89     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TATTCACGACGGACCCTCCT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1019     End: 1357     Product size (bp): 339
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAG)3
Repeat start & end within CDS Repeat start: 1378     Repeat end: 1386
Forward primer Primer sequence:   AGGAGGGTCCGTCGTGAATA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGCCTGTAAGCATTCCCTT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1338     End: 1681     Product size (bp): 344
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCCAA)2
Repeat start & end within CDS Repeat start: 1603     Repeat end: 1612
Forward primer Primer sequence:   AGGAGGGTCCGTCGTGAATA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGCCTGTAAGCATTCCCTT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1338     End: 1681     Product size (bp): 344
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGAAG)2ccagccctcagtaacatggt(TGC)3
Repeat start & end within CDS Repeat start: 2223     Repeat end: 2261
Forward primer Primer sequence:   TGAAGTACGGTGCTGGCATT     Tm(°C): 59.964     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CACTTGGTACTGGCGAGCTG     Tm(°C): 61.016     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 2059     End: 2296     Product size (bp): 238
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Enzyme Id:  K00549

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335992     NCBI Gene Symbol: LOC103335992
Gene Aliases
Gene description & Other designations Description:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase      Other designations:   5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 14691779 ...... 14696859
CDS Sequence 103335992
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGGAGGGTCCGTCGTGAATA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGCCTGTAAGCATTCCCTT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1338     End: 1681     Product size (bp): 344
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