Statistics
Number of enzymes: 1
Total Number of designed primers: 9
Number of PGTM primers: 2
Number of PMTM primers: 7
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103330432 NCBI Gene Symbol: LOC103330432 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG4 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024129.1 Gene Start and end within genomic accession: 23892015 ...... 23894085 |
| CDS Sequence | 103330432 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Compound Repeat sequence: (TCTGT)2gtctgcgtcgccatggaggcccaatcaggccgccaccgcatcctcct
(CTGTC)2 |
| Repeat start & end within CDS | Repeat start: 26 Repeat end: 92 |
| Forward primer | Primer sequence: TTGAGGGTTGCGGCGAG Tm(°C): 60.012 GC (%): 64.706 Size: 17 |
| Reverse primer | Primer sequence: ATGGATCTGAGGTCGTCGGA Tm(°C): 60.107 GC (%): 55 Size: 20 |
| Primer start, end within sequence and product size | Start: 3 End: 139 Product size (bp): 137 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103330432 NCBI Gene Symbol: LOC103330432 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG4 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024129.1 Gene Start and end within genomic accession: 23892015 ...... 23894085 |
| CDS Sequence | 103330432 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Hexa Repeat sequence: (ACTGTG)2 |
| Repeat start & end within CDS | Repeat start: 484 Repeat end: 495 |
| Forward primer | Primer sequence: CGCTCGAGGTTGATCTCTCC Tm(°C): 59.97 GC (%): 60 Size: 20 |
| Reverse primer | Primer sequence: ATGTGTGCAGACCCCAACAA Tm(°C): 60.106 GC (%): 50 Size: 20 |
| Primer start, end within sequence and product size | Start: 445 End: 650 Product size (bp): 206 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103330432 NCBI Gene Symbol: LOC103330432 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG4 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024129.1 Gene Start and end within genomic accession: 23892015 ...... 23894085 |
| CDS Sequence | 103330432 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Tri Repeat sequence: (TTG)3 |
| Repeat start & end within CDS | Repeat start: 629 Repeat end: 637 |
| Forward primer | Primer sequence: CGCTCGAGGTTGATCTCTCC Tm(°C): 59.97 GC (%): 60 Size: 20 |
| Reverse primer | Primer sequence: AACCCAGATGTGTGCAGACC Tm(°C): 60.251 GC (%): 55 Size: 20 |
| Primer start, end within sequence and product size | Start: 445 End: 657 Product size (bp): 213 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103330432 NCBI Gene Symbol: LOC103330432 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG4 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024129.1 Gene Start and end within genomic accession: 23892015 ...... 23894085 |
| CDS Sequence | 103330432 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Penta Repeat sequence: (CCATG)2 |
| Repeat start & end within CDS | Repeat start: 703 Repeat end: 712 |
| Forward primer | Primer sequence: CGCTCGAGGTTGATCTCTCC Tm(°C): 59.97 GC (%): 60 Size: 20 |
| Reverse primer | Primer sequence: CCTTGCGGATCCTTCCAGAA Tm(°C): 59.747 GC (%): 55 Size: 20 |
| Primer start, end within sequence and product size | Start: 445 End: 747 Product size (bp): 303 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103330432 NCBI Gene Symbol: LOC103330432 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG4 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024129.1 Gene Start and end within genomic accession: 23892015 ...... 23894085 |
| CDS Sequence | 103330432 |
| Marker Information | |
| Marker Type | PGTM |
| Repeat type & sequence | Repeat type: Repeat sequence: |
| Repeat start & end within CDS | Repeat start: Repeat end: |
| Forward primer | Primer sequence: AGGCTCTGTTCTGTGTCTGC Tm(°C): 59.965 GC (%): 55 Size: 20 |
| Reverse primer | Primer sequence: GGAGAGATCAACCTCGAGCG Tm(°C): 59.97 GC (%): 60 Size: 20 |
| Primer start, end within sequence and product size | Start: 21 End: 464 Product size (bp): 444 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103322148 NCBI Gene Symbol: LOC103322148 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG2 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024127.1 Gene Start and end within genomic accession: 21430637 ...... 21432820 |
| CDS Sequence | 103322148 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Compound Repeat sequence: (CCTTGC)2ttcccttgaggttgatctgtccagcattgagcctggtac(TACTGT
)2 |
| Repeat start & end within CDS | Repeat start: 444 Repeat end: 506 |
| Forward primer | Primer sequence: CCTTGATCCGGCTTCTGGTT Tm(°C): 60.035 GC (%): 55 Size: 20 |
| Reverse primer | Primer sequence: AGTGCAGACCCCAACAACAA Tm(°C): 60.034 GC (%): 50 Size: 20 |
| Primer start, end within sequence and product size | Start: 385 End: 659 Product size (bp): 275 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103322148 NCBI Gene Symbol: LOC103322148 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG2 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024127.1 Gene Start and end within genomic accession: 21430637 ...... 21432820 |
| CDS Sequence | 103322148 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Tri Repeat sequence: (TTG)4 |
| Repeat start & end within CDS | Repeat start: 638 Repeat end: 649 |
| Forward primer | Primer sequence: GTGAAGTGGAGAGGGAAGCC Tm(°C): 60.036 GC (%): 60 Size: 20 |
| Reverse primer | Primer sequence: TTGCGGATCCTGCCAGAAAT Tm(°C): 60.034 GC (%): 50 Size: 20 |
| Primer start, end within sequence and product size | Start: 504 End: 757 Product size (bp): 254 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103322148 NCBI Gene Symbol: LOC103322148 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG2 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024127.1 Gene Start and end within genomic accession: 21430637 ...... 21432820 |
| CDS Sequence | 103322148 |
| Marker Information | |
| Marker Type | PMTM |
| Repeat type & sequence | Repeat type: Penta Repeat sequence: (CCATG)2 |
| Repeat start & end within CDS | Repeat start: 715 Repeat end: 724 |
| Forward primer | Primer sequence: TTGTTGTTGGGGTCTGCACT Tm(°C): 60.034 GC (%): 50 Size: 20 |
| Reverse primer | Primer sequence: TTGCGGATCCTGCCAGAAAT Tm(°C): 60.034 GC (%): 50 Size: 20 |
| Primer start, end within sequence and product size | Start: 640 End: 757 Product size (bp): 118 |
| JBrowse View | JBrowse |
|
| Gene Information | |
| NCBI Gene Accession Number & Symbol | Accession Number: 103322148 NCBI Gene Symbol: LOC103322148 |
| Gene Aliases | |
| Gene description & Other designations | Description: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like Other designations: cytochrome b-c1 complex subunit Rieske-4; mitochondrial-like |
| Chromosome, Strand & Exon count | Chromosome: LG2 Strand: plus Exon count: 2 |
| Gene Location within genomic sequence | Genomic accession No. NC_024127.1 Gene Start and end within genomic accession: 21430637 ...... 21432820 |
| CDS Sequence | 103322148 |
| Marker Information | |
| Marker Type | PGTM |
| Repeat type & sequence | Repeat type: Repeat sequence: |
| Repeat start & end within CDS | Repeat start: Repeat end: |
| Forward primer | Primer sequence: CGACGACTCTTCCTCCGATG Tm(°C): 59.972 GC (%): 60 Size: 20 |
| Reverse primer | Primer sequence: AACCAGAAGCCGGATCAAGG Tm(°C): 60.035 GC (%): 55 Size: 20 |
| Primer start, end within sequence and product size | Start: 107 End: 404 Product size (bp): 298 |
| JBrowse View | JBrowse |
