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Statistics

Number of enzymes: 1
Total Number of designed primers: 6
Number of PGTM primers:     2
Number of PMTM primers:     4
Number of Failed designed PMTM primers: 1

Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340476     NCBI Gene Symbol: LOC103340476
Gene Aliases
Gene description & Other designations Description:   thioredoxin reductase NTRB-like      Other designations:   thioredoxin reductase NTRB-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 12709823 ...... 12712506
CDS Sequence 103340476
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCG)3
Repeat start & end within CDS Repeat start: 223     Repeat end: 231
Forward primer Primer sequence:   CAAGGTGCTGAGTCTGCTGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCCATCCAGCCCTCGAATAG     Tm(°C): 60.321     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 23     End: 283     Product size (bp): 261
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340476     NCBI Gene Symbol: LOC103340476
Gene Aliases
Gene description & Other designations Description:   thioredoxin reductase NTRB-like      Other designations:   thioredoxin reductase NTRB-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 12709823 ...... 12712506
CDS Sequence 103340476
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CAC)4
Repeat start & end within CDS Repeat start: 312     Repeat end: 323
Forward primer Primer sequence:   CGATCCTATTCGAGGGCTGG     Tm(°C): 59.755     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GTTGCCACAACAACGGAGTC     Tm(°C): 59.971     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 259     End: 517     Product size (bp): 259
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340476     NCBI Gene Symbol: LOC103340476
Gene Aliases
Gene description & Other designations Description:   thioredoxin reductase NTRB-like      Other designations:   thioredoxin reductase NTRB-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 12709823 ...... 12712506
CDS Sequence 103340476
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTG)3
Repeat start & end within CDS Repeat start: 1073     Repeat end: 1081
Forward primer Primer sequence:   ACGAACCGGCGACTAAGTTT     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATGTTCTGCTTCCAAGGCCG     Tm(°C): 60.962     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 925     End: 1115     Product size (bp): 191
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340476     NCBI Gene Symbol: LOC103340476
Gene Aliases
Gene description & Other designations Description:   thioredoxin reductase NTRB-like      Other designations:   thioredoxin reductase NTRB-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 12709823 ...... 12712506
CDS Sequence 103340476
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (ACCCAA)2acaaggtgctgagtctgctgagaagagct(CGCAG)2ttggggt(A
GC)4ttcgttttcgtcattgc(CTTCGT)2
Repeat start & end within CDS Repeat start: 11     Repeat end: 109
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340476     NCBI Gene Symbol: LOC103340476
Gene Aliases
Gene description & Other designations Description:   thioredoxin reductase NTRB-like      Other designations:   thioredoxin reductase NTRB-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 12709823 ...... 12712506
CDS Sequence 103340476
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GACTCCGTTGTTGTGGCAAC     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AAACTTAGTCGCCGGTTCGT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 498     End: 944     Product size (bp): 447
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342404     NCBI Gene Symbol: LOC103342404
Gene Aliases
Gene description & Other designations Description:   NADPH-dependent thioredoxin reductase 3      Other designations:   NADPH-dependent thioredoxin reductase 3
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342404
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CT)4(CTCTCC)2*(CCTCT)2*ctcctccacgctcacgccctcact(CTCTCC
)2
Repeat start & end within CDS Repeat start: 139     Repeat end: 193
Forward primer Primer sequence:   GTTTCGAGACCGTTCCGACT     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGCTGGACCTGACCCAATT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 31     End: 270     Product size (bp): 240
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Enzyme Id:  K00384
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342404     NCBI Gene Symbol: LOC103342404
Gene Aliases
Gene description & Other designations Description:   NADPH-dependent thioredoxin reductase 3      Other designations:   NADPH-dependent thioredoxin reductase 3
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342404
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTTTCGAGACCGTTCCGACT     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGCTGGACCTGACCCAATT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 31     End: 270     Product size (bp): 240
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