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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5
Number of Failed designed PMTM primers: 1

Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323127     NCBI Gene Symbol: LOC103323127
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase 1; chloroplastic-like      Other designations:   dihydrolipoyl dehydrogenase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 32273013 ...... 32277695
CDS Sequence 103323127
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GTCTCG)2
Repeat start & end within CDS Repeat start: 184     Repeat end: 195
Forward primer Primer sequence:   GAGTCTCTGCTCCTTTGCGT     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCACCCACCACATCTCCTTC     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 154     End: 343     Product size (bp): 190
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323127     NCBI Gene Symbol: LOC103323127
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase 1; chloroplastic-like      Other designations:   dihydrolipoyl dehydrogenase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 32273013 ...... 32277695
CDS Sequence 103323127
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGGA)2
Repeat start & end within CDS Repeat start: 1076     Repeat end: 1085
Forward primer Primer sequence:   GCCCCATTCACACAAGGTCT     Tm(°C): 60.251     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCTGCGGGGATGCTTAAATG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1056     End: 1300     Product size (bp): 245
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323127     NCBI Gene Symbol: LOC103323127
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase 1; chloroplastic-like      Other designations:   dihydrolipoyl dehydrogenase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 32273013 ...... 32277695
CDS Sequence 103323127
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 13     Repeat end: 20
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323127     NCBI Gene Symbol: LOC103323127
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase 1; chloroplastic-like      Other designations:   dihydrolipoyl dehydrogenase 1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 32273013 ...... 32277695
CDS Sequence 103323127
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CATTTAAGCATCCCCGCAGC     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGGTCTGCAGCATGTAACCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1281     End: 1525     Product size (bp): 245
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323235     NCBI Gene Symbol: LOC103323235
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase; mitochondrial      Other designations:   dihydrolipoyl dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 33105670 ...... 33107994
CDS Sequence 103323235
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CGT)3
Repeat start & end within CDS Repeat start: 144     Repeat end: 152
Forward primer Primer sequence:   TGTCCAACACGTCATCGGAG     Tm(°C): 60.039     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTTGGAGGGTATGCAGCCAA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 52     End: 281     Product size (bp): 230
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323235     NCBI Gene Symbol: LOC103323235
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase; mitochondrial      Other designations:   dihydrolipoyl dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 33105670 ...... 33107994
CDS Sequence 103323235
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GACCA)2
Repeat start & end within CDS Repeat start: 904     Repeat end: 913
Forward primer Primer sequence:   CAGTGTGGCGTAGACTTGGT     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGAACCCTACCAGCAGACAC     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 700     End: 955     Product size (bp): 256
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323235     NCBI Gene Symbol: LOC103323235
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase; mitochondrial      Other designations:   dihydrolipoyl dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 33105670 ...... 33107994
CDS Sequence 103323235
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGATA)2
Repeat start & end within CDS Repeat start: 1347     Repeat end: 1356
Forward primer Primer sequence:   TACGCACCCTGAAGTTGCAT     Tm(°C): 59.964     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAGCTTCCTTCAAGGCCTCA     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1187     End: 1497     Product size (bp): 311
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Enzyme Id:  K00382

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323235     NCBI Gene Symbol: LOC103323235
Gene Aliases
Gene description & Other designations Description:   dihydrolipoyl dehydrogenase; mitochondrial      Other designations:   dihydrolipoyl dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 33105670 ...... 33107994
CDS Sequence 103323235
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTGGCTGCATACCCTCCAAG     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCAAGTCTACGCCACACTG     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 262     End: 719     Product size (bp): 458
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