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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K00207

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   109823934     NCBI Gene Symbol: LOC109823934
Gene Aliases A4U43_C09F14410
Gene description & Other designations Description:   dihydropyrimidine dehydrogenase (NADP(+)); chloroplastic      Other designations:   dihydropyrimidine dehydrogenase (NADP(+)); chloroplastic
Chromosome, Strand & Exon count Chromosome:   9     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_033802.1      Gene Start and end within genomic accession: 61673646 ...... 61680658
CDS Sequence 109823934
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCGGG)2
Repeat start & end within CDS Repeat start: 20     Repeat end: 29
Forward primer Primer sequence:   ATGCCGAACCCGTTTGTTAT     Tm(°C): 58.171     GC (%): 45     Size: 20
Reverse primer Primer sequence:   ATTTGATCCCGCTGCTCGAA     Tm(°C): 60.108     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 0     End: 173     Product size (bp): 174
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Enzyme Id:  K00207

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   109823934     NCBI Gene Symbol: LOC109823934
Gene Aliases A4U43_C09F14410
Gene description & Other designations Description:   dihydropyrimidine dehydrogenase (NADP(+)); chloroplastic      Other designations:   dihydropyrimidine dehydrogenase (NADP(+)); chloroplastic
Chromosome, Strand & Exon count Chromosome:   9     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_033802.1      Gene Start and end within genomic accession: 61673646 ...... 61680658
CDS Sequence 109823934
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAGAGGGCGTTTGATGAGGG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCATCCACTCCAGTCTCCT     Tm(°C): 59.958     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 57     End: 374     Product size (bp): 318
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