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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     2
Number of PMTM primers:     2

Enzyme Id:  K00162

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322229     NCBI Gene Symbol: LOC103322229
Gene Aliases
Gene description & Other designations Description:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial      Other designations:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 22136904 ...... 22142035
CDS Sequence 103322229
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TGC)3
Repeat start & end within CDS Repeat start: 477     Repeat end: 485
Forward primer Primer sequence:   CTGGGATTGGAGTTGGTGCT     Tm(°C): 59.961     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGTCCCTAATGGCAGCTTT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 307     End: 610     Product size (bp): 304
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Enzyme Id:  K00162

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322229     NCBI Gene Symbol: LOC103322229
Gene Aliases
Gene description & Other designations Description:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial      Other designations:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 22136904 ...... 22142035
CDS Sequence 103322229
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGGA)2
Repeat start & end within CDS Repeat start: 728     Repeat end: 737
Forward primer Primer sequence:   AAAGCTGCCATTAGGGACCC     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACCATGCTGAGGAAACCCT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 591     End: 930     Product size (bp): 340
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Enzyme Id:  K00162

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322229     NCBI Gene Symbol: LOC103322229
Gene Aliases
Gene description & Other designations Description:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial      Other designations:   pyruvate dehydrogenase E1 component subunit beta-1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 22136904 ...... 22142035
CDS Sequence 103322229
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGTCTGCAGACCCGAAAGTC     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGTCCCTAATGGCAGCTTT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 169     End: 610     Product size (bp): 442
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Enzyme Id:  K00162

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103325136     NCBI Gene Symbol: LOC103325136
Gene Aliases
Gene description & Other designations Description:   pyruvate dehydrogenase E1 component subunit beta-3; chloroplastic-like      Other designations:   pyruvate dehydrogenase E1 component subunit beta-3; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 4398855 ...... 4401534
CDS Sequence 103325136
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATGGCTAGGGATCCCACTGT     Tm(°C): 60.03     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AAGGGGTTGAGCATGCTACC     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 297     End: 699     Product size (bp): 403
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