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Statistics

Number of enzymes: 1
Total Number of designed primers: 8
Number of PGTM primers:     2
Number of PMTM primers:     6

Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339579     NCBI Gene Symbol: LOC103339579
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7679186 ...... 7689526
CDS Sequence 103339579
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGTCA)2
Repeat start & end within CDS Repeat start: 456     Repeat end: 465
Forward primer Primer sequence:   AAAGGCTCGCACAAGGAAGT     Tm(°C): 60.179     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGCAAACACCTCCATTGGGA     Tm(°C): 59.813     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 270     End: 552     Product size (bp): 283
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339579     NCBI Gene Symbol: LOC103339579
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7679186 ...... 7689526
CDS Sequence 103339579
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTGCTA)2
Repeat start & end within CDS Repeat start: 989     Repeat end: 1000
Forward primer Primer sequence:   CGAAAGAGTGCTGCAGGGTA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACGGGAATATGCCTCTGTGC     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 831     End: 1142     Product size (bp): 312
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339579     NCBI Gene Symbol: LOC103339579
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   19
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7679186 ...... 7689526
CDS Sequence 103339579
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGAAAGAGTGCTGCAGGGTA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTAGGCTCCATCGCAAAGCA     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 831     End: 1282     Product size (bp): 452
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339581     NCBI Gene Symbol: LOC103339581
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7720047 ...... 7727911
CDS Sequence 103339581
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCGCAG)2
Repeat start & end within CDS Repeat start: 176     Repeat end: 187
Forward primer Primer sequence:   CTCGCTGCTTCCAATTGCTC     Tm(°C): 59.902     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGGGGAAAACAACCACGTCA     Tm(°C): 60.034     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 155     End: 429     Product size (bp): 275
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339581     NCBI Gene Symbol: LOC103339581
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7720047 ...... 7727911
CDS Sequence 103339581
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGTCA)2
Repeat start & end within CDS Repeat start: 456     Repeat end: 465
Forward primer Primer sequence:   TGACGTGGTTGTTTTCCCCA     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTCCAATTGTTGCACCAGGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 410     End: 708     Product size (bp): 299
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339581     NCBI Gene Symbol: LOC103339581
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7720047 ...... 7727911
CDS Sequence 103339581
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tetra     Repeat sequence: (TGGA)3
Repeat start & end within CDS Repeat start: 627     Repeat end: 638
Forward primer Primer sequence:   TGACGTGGTTGTTTTCCCCA     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTCCAATTGTTGCACCAGGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 410     End: 708     Product size (bp): 299
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339581     NCBI Gene Symbol: LOC103339581
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7720047 ...... 7727911
CDS Sequence 103339581
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTGCTA)2
Repeat start & end within CDS Repeat start: 989     Repeat end: 1000
Forward primer Primer sequence:   CGAAAGAGTGCTGCAGGGTA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGATGGCCCTCACTTGAACC     Tm(°C): 59.962     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 831     End: 1065     Product size (bp): 235
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Enzyme Id:  K00102

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103339581     NCBI Gene Symbol: LOC103339581
Gene Aliases
Gene description & Other designations Description:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like      Other designations:   D-lactate dehydrogenase [cytochrome]; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   18
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 7720047 ...... 7727911
CDS Sequence 103339581
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGACGTGGTTGTTTTCCCCA     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TACCCTGCAGCACTCTTTCG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 410     End: 850     Product size (bp): 441
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