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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K00051

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331480     NCBI Gene Symbol: LOC103331480
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase [NADP]; chloroplastic      Other designations:   malate dehydrogenase [NADP]; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12202560 ...... 12208112
CDS Sequence 103331480
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 262     Repeat end: 270
Forward primer Primer sequence:   CCGCTCCCTCAGGCTAAAAA     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGGTCTGGCCCAAAAACCT     Tm(°C): 60.032     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 114     End: 380     Product size (bp): 267
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Enzyme Id:  K00051

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331480     NCBI Gene Symbol: LOC103331480
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase [NADP]; chloroplastic      Other designations:   malate dehydrogenase [NADP]; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12202560 ...... 12208112
CDS Sequence 103331480
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGAA)2
Repeat start & end within CDS Repeat start: 561     Repeat end: 570
Forward primer Primer sequence:   GTTGTTGAGGGAGGTCAGCA     Tm(°C): 59.891     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCTTTGAGGGCTAGCTGACA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 467     End: 790     Product size (bp): 324
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Enzyme Id:  K00051

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331480     NCBI Gene Symbol: LOC103331480
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase [NADP]; chloroplastic      Other designations:   malate dehydrogenase [NADP]; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12202560 ...... 12208112
CDS Sequence 103331480
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GGA)3
Repeat start & end within CDS Repeat start: 924     Repeat end: 932
Forward primer Primer sequence:   GTCAGCTAGCCCTCAAAGCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCAATTGACACGGCAGTTG     Tm(°C): 59.969     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 772     End: 1019     Product size (bp): 248
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Enzyme Id:  K00051

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331480     NCBI Gene Symbol: LOC103331480
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase [NADP]; chloroplastic      Other designations:   malate dehydrogenase [NADP]; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   14
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 12202560 ...... 12208112
CDS Sequence 103331480
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTCAGCTAGCCCTCAAAGCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCAATTGACACGGCAGTTG     Tm(°C): 59.969     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 772     End: 1019     Product size (bp): 248
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