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Statistics

Number of enzymes: 1
Total Number of designed primers: 15
Number of PGTM primers:     3
Number of PMTM primers:     12

Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340826     NCBI Gene Symbol: LOC103340826
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14647283 ...... 14652409
CDS Sequence 103340826
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTGTAA)2
Repeat start & end within CDS Repeat start: 168     Repeat end: 179
Forward primer Primer sequence:   GCTCCAGCGATCACTCTGTT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACTCCTTGAAGGCGCTCTC     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 140     End: 250     Product size (bp): 111
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340826     NCBI Gene Symbol: LOC103340826
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14647283 ...... 14652409
CDS Sequence 103340826
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGGT)2
Repeat start & end within CDS Repeat start: 1733     Repeat end: 1742
Forward primer Primer sequence:   TTTCCAACCCGACGTCTCAG     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCATTGTGCACACGGATTGC     Tm(°C): 59.759     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1564     End: 1771     Product size (bp): 208
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340826     NCBI Gene Symbol: LOC103340826
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   minus     Exon count:   20
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14647283 ...... 14652409
CDS Sequence 103340826
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GACAGAGCTCCAGGAGAGGA     Tm(°C): 60.034     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TACCGGAATTCCCATCCCCT     Tm(°C): 60.029     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 566     End: 854     Product size (bp): 289
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CCACA)2acaaaggtcttgccttcacagagaa(AG)4
Repeat start & end within CDS Repeat start: 325     Repeat end: 367
Forward primer Primer sequence:   CCCTGGAGTGTCTCTGTTGC     Tm(°C): 60.321     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TTTCTGGCAAGCTTCACCGA     Tm(°C): 60.179     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 279     End: 596     Product size (bp): 318
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ATGGG)2
Repeat start & end within CDS Repeat start: 755     Repeat end: 764
Forward primer Primer sequence:   CACTGATGGAGAGCGGATCC     Tm(°C): 59.967     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AGCCCTCTTTTGCCTGAGTC     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 710     End: 908     Product size (bp): 199
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CATGAA)2
Repeat start & end within CDS Repeat start: 1333     Repeat end: 1344
Forward primer Primer sequence:   GGCTTGTGGACTCAAAGGGA     Tm(°C): 59.89     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTGCTCCTGACGTTCCAAT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1258     End: 1414     Product size (bp): 157
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCAAC)2
Repeat start & end within CDS Repeat start: 1487     Repeat end: 1496
Forward primer Primer sequence:   ATTGGAACGTCAGGAGCAGG     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AATGCTTCCGAGGCTGCTAG     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1395     End: 1717     Product size (bp): 323
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTTGG)2
Repeat start & end within CDS Repeat start: 1645     Repeat end: 1654
Forward primer Primer sequence:   ATTGGAACGTCAGGAGCAGG     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AATGCTTCCGAGGCTGCTAG     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1395     End: 1717     Product size (bp): 323
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327837     NCBI Gene Symbol: LOC103327837
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme 4; chloroplastic      Other designations:   NADP-dependent malic enzyme 4; chloroplastic|NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 4011309 ...... 4017927
CDS Sequence 103327837
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTTGGTCCCAAAAACCCGGA     Tm(°C): 60.032     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GGACGGTGGATTTCAGCTCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 110     End: 219     Product size (bp): 110
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 280     Repeat end: 287
Forward primer Primer sequence:   GGTCCTCACACCATGGACTG     Tm(°C): 60.036     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCAAGCCTCACCAACTGTTG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 188     End: 509     Product size (bp): 322
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 561     Repeat end: 568
Forward primer Primer sequence:   CAACAGTTGGTGAGGCTTGC     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCTCTCCGGCCAATTCTTT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 490     End: 609     Product size (bp): 120
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CTGGAA)2
Repeat start & end within CDS Repeat start: 1094     Repeat end: 1105
Forward primer Primer sequence:   CAGGGAACAGCATCTGTGGT     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACGGCGTCTACAAGTTCCT     Tm(°C): 60.039     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 990     End: 1289     Product size (bp): 300
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCAAC)2
Repeat start & end within CDS Repeat start: 1406     Repeat end: 1415
Forward primer Primer sequence:   GGCTCATGACCATGAACCCA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCCTTGGTCCATGCGTAAG     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1247     End: 1463     Product size (bp): 217
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGCCA)2
Repeat start & end within CDS Repeat start: 1531     Repeat end: 1540
Forward primer Primer sequence:   CTTACGCATGGACCAAGGGT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTAACTTGGGCAGCCAAAGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1444     End: 1651     Product size (bp): 208
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Enzyme Id:  K00029
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320247     NCBI Gene Symbol: LOC103320247
Gene Aliases
Gene description & Other designations Description:   NADP-dependent malic enzyme      Other designations:   NADP-dependent malic enzyme
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   21
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 9663848 ...... 9668741
CDS Sequence 103320247
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAAAGCAAGGCTGGGTTTGG     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCAAGCCTCACCAACTGTTG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 120     End: 509     Product size (bp): 390
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