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Statistics

Number of enzymes: 1
Total Number of designed primers: 15
Number of PGTM primers:     5
Number of PMTM primers:     10

Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103338505     NCBI Gene Symbol: LOC103338505
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; chloroplastic      Other designations:   malate dehydrogenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 14949427 ...... 14951580
CDS Sequence 103338505
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AAG)3
Repeat start & end within CDS Repeat start: 679     Repeat end: 687
Forward primer Primer sequence:   GTCCAGAGGCCTTCATCCAC     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CACCAGCCTTTGCCTCTACA     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 607     End: 942     Product size (bp): 336
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103338505     NCBI Gene Symbol: LOC103338505
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; chloroplastic      Other designations:   malate dehydrogenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 14949427 ...... 14951580
CDS Sequence 103338505
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AGA)3atctaaaactga(TTGATG)2
Repeat start & end within CDS Repeat start: 761     Repeat end: 793
Forward primer Primer sequence:   GTCCAGAGGCCTTCATCCAC     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CACCAGCCTTTGCCTCTACA     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 607     End: 942     Product size (bp): 336
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103338505     NCBI Gene Symbol: LOC103338505
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; chloroplastic      Other designations:   malate dehydrogenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 14949427 ...... 14951580
CDS Sequence 103338505
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCTGA)2
Repeat start & end within CDS Repeat start: 1051     Repeat end: 1060
Forward primer Primer sequence:   GGTGCTGGGTCTGCTACATT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTCACTGCTTGCTTGTTGGC     Tm(°C): 60.596     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 939     End: 1237     Product size (bp): 299
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103338505     NCBI Gene Symbol: LOC103338505
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; chloroplastic      Other designations:   malate dehydrogenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 14949427 ...... 14951580
CDS Sequence 103338505
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCACAGTCAAAGCCTTGTGC     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGGTGGTTTCTTTGCCCAAG     Tm(°C): 59.968     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 75     End: 204     Product size (bp): 130
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335687     NCBI Gene Symbol: LOC103335687
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; glyoxysomal      Other designations:   malate dehydrogenase; glyoxysomal
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 18590473 ...... 18593606
CDS Sequence 103335687
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GATGAA)2
Repeat start & end within CDS Repeat start: 195     Repeat end: 206
Forward primer Primer sequence:   TCCACATGGAGAACACTGCC     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACCAAATCCACTCCGGTCA     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 73     End: 353     Product size (bp): 281
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335687     NCBI Gene Symbol: LOC103335687
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; glyoxysomal      Other designations:   malate dehydrogenase; glyoxysomal
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 18590473 ...... 18593606
CDS Sequence 103335687
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCA)3
Repeat start & end within CDS Repeat start: 312     Repeat end: 320
Forward primer Primer sequence:   CGTCACCTCCGATATCAGCC     Tm(°C): 60.039     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACGTTGACAATGGCGTTTGG     Tm(°C): 59.97     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 254     End: 481     Product size (bp): 228
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335687     NCBI Gene Symbol: LOC103335687
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; glyoxysomal      Other designations:   malate dehydrogenase; glyoxysomal
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 18590473 ...... 18593606
CDS Sequence 103335687
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AGCTGG)2
Repeat start & end within CDS Repeat start: 789     Repeat end: 800
Forward primer Primer sequence:   AGCCTCCATGCTCTTTCACC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCCGTACCTTGGATGCAAA     Tm(°C): 59.962     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 703     End: 946     Product size (bp): 244
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335687     NCBI Gene Symbol: LOC103335687
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; glyoxysomal      Other designations:   malate dehydrogenase; glyoxysomal
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 18590473 ...... 18593606
CDS Sequence 103335687
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCAAACGCCATTGTCAACGT     Tm(°C): 59.97     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAGCTTTGGCCTCAACAACC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 462     End: 792     Product size (bp): 331
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324868     NCBI Gene Symbol: LOC103324868
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial-like      Other designations:   malate dehydrogenase; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 2695782 ...... 2697677
CDS Sequence 103324868
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CTCCCT)2
Repeat start & end within CDS Repeat start: 159     Repeat end: 170
Forward primer Primer sequence:   ACTGTCGCTCCTCATGAAGC     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCGCTCAAAGACTTGACGA     Tm(°C): 59.968     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 122     End: 383     Product size (bp): 262
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324868     NCBI Gene Symbol: LOC103324868
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial-like      Other designations:   malate dehydrogenase; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 2695782 ...... 2697677
CDS Sequence 103324868
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TAAGG)2
Repeat start & end within CDS Repeat start: 879     Repeat end: 888
Forward primer Primer sequence:   TTGAAGGGTCTAAACGGGGC     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCAAGAACTTCCTCCACGC     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 795     End: 920     Product size (bp): 126
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103324868     NCBI Gene Symbol: LOC103324868
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial-like      Other designations:   malate dehydrogenase; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 2695782 ...... 2697677
CDS Sequence 103324868
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTCCCTCTCCCTGTACGACA     Tm(°C): 60.034     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AGCGCTCAAAGACTTGACGA     Tm(°C): 59.968     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 158     End: 383     Product size (bp): 226
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326072     NCBI Gene Symbol: LOC103326072
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial      Other designations:   malate dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 10944389 ...... 10947461
CDS Sequence 103326072
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)5
Repeat start & end within CDS Repeat start: 163     Repeat end: 172
Forward primer Primer sequence:   CATGTCCCTCGTCAGATCCG     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ATGATTACGACGTCGGCTCC     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 11     End: 298     Product size (bp): 288
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326072     NCBI Gene Symbol: LOC103326072
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial      Other designations:   malate dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 10944389 ...... 10947461
CDS Sequence 103326072
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AATGTT)2
Repeat start & end within CDS Repeat start: 562     Repeat end: 573
Forward primer Primer sequence:   AGTCAACTCTACGGTCCCGA     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCAGATCCCTTTCCAGCCTT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 434     End: 745     Product size (bp): 312
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103326072     NCBI Gene Symbol: LOC103326072
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; mitochondrial      Other designations:   malate dehydrogenase; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 10944389 ...... 10947461
CDS Sequence 103326072
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CATGTCCCTCGTCAGATCCG     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ATGATTACGACGTCGGCTCC     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 11     End: 298     Product size (bp): 288
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Enzyme Id:  K00026

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334965     NCBI Gene Symbol: LOC103334965
Gene Aliases
Gene description & Other designations Description:   malate dehydrogenase; glyoxysomal      Other designations:   malate dehydrogenase; glyoxysomal
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 6182719 ...... 6185471
CDS Sequence 103334965
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGCATTGCAAGGATCTCAGC     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCACCCAAAATCGCCACTTT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 27     End: 154     Product size (bp): 128
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